Binding of Different Dimeric Forms of PDGF to Human Fibroblasts: Evidence for Two Separate Receptor Types

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1988 May 1

PMID 2842148

Citations 108

Authors

C H Heldin

G Backstrom

A Ostman

A Hammacher

L Ronnstrand

K Rubin

M Nister

B Westermark

Affiliations

Soon will be listed here.

Abstract

The binding of the three dimeric forms of platelet-derived growth factor (PDGF), PDGF-AA, PDGF-AB and PDGF-BB, to human fibroblasts was studied. Cross-competition experiments revealed the existence of two different PDGF receptor classes: the type A PDGF receptor bound all three dimeric forms of PDGF, whereas the type B PDGF receptor bound PDGF-BB with high affinity and PDGF-AB with lower affinity, but not PDGF-AA. The sizes of the two receptors were estimated with affinity labeling techniques; the A type receptor appeared as a major component of 125 kd and a minor of 160 kd, and the B type receptor as two components of 160 and 175 kd. A previously established PDGF receptor monoclonal antibody, PDGFR-B2, was shown to react with the B type receptor only. The different abilities of the three dimeric forms of PDGF to stimulate incorporation of [3H]TdR into human fibroblasts indicated that the major mitogenic effect of PDGF is mediated via the B type receptor.

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