Structural Basis of Stringent PAM Recognition by CRISPR-C2c1 in Complex with SgRNA

Overview

Journal Cell Res

Specialty Cell Biology

Date 2017 Apr 5

PMID 28374750

Citations 24

Authors

Dan Wu

Xiaoyu Guan

Yuwei Zhu

Kuan Ren

Zhiwei Huang

Affiliations

Soon will be listed here.

Citing Articles

Exploring the Thermostability of CRISPR-Cas12b using Molecular Dynamics Simulations.

Jia Y, Horvath K, Rananaware S, Jain P, Sampath J ArXiv. 2024; .

PMID: 39253640 PMC: 11383325.

TracrRNA reprogramming enables direct PAM-independent detection of RNA with diverse DNA-targeting Cas12 nucleases.

Jiao C, Peeck N, Yu J, Ghaem Maghami M, Kono S, Collias D Nat Commun. 2024; 15(1):5909.

PMID: 39003282 PMC: 11246509. DOI: 10.1038/s41467-024-50243-x.

A New-Generation Base Editor with an Expanded Editing Window for Microbial Cell Evolution In Vivo Based on CRISPR‒Cas12b Engineering.

Hao W, Cui W, Liu Z, Suo F, Wu Y, Han L Adv Sci (Weinh). 2024; 11(22):e2309767.

PMID: 38602436 PMC: 11165516. DOI: 10.1002/advs.202309767.

Application of CRISPR-Cas system in the diagnosis and therapy of ESKAPE infections.

Qian Y, Zhou D, Li M, Zhao Y, Liu H, Yang L Front Cell Infect Microbiol. 2023; 13:1223696.

PMID: 37662004 PMC: 10470840. DOI: 10.3389/fcimb.2023.1223696.

Engineering highly thermostable Cas12b via de novo structural analyses for one-pot detection of nucleic acids.

Nguyen L, Rananaware S, Yang L, Macaluso N, Ocana-Ortiz J, Meister K Cell Rep Med. 2023; 4(5):101037.

PMID: 37160120 PMC: 10213852. DOI: 10.1016/j.xcrm.2023.101037.

References

Shmakov S, Abudayyeh O, Makarova K, Wolf Y, Gootenberg J, Semenova E . Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Mol Cell. 2015; 60(3):385-97. PMC: 4660269. DOI: 10.1016/j.molcel.2015.10.008. View

Nishimasu H, Cong L, Yan W, Ran F, Zetsche B, Li Y . Crystal Structure of Staphylococcus aureus Cas9. Cell. 2015; 162(5):1113-26. PMC: 4670267. DOI: 10.1016/j.cell.2015.08.007. View

Makarova K, Wolf Y, Alkhnbashi O, Costa F, Shah S, Saunders S . An updated evolutionary classification of CRISPR-Cas systems. Nat Rev Microbiol. 2015; 13(11):722-36. PMC: 5426118. DOI: 10.1038/nrmicro3569. View

Yang H, Gao P, Rajashankar K, Patel D . PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease. Cell. 2016; 167(7):1814-1828.e12. PMC: 5278635. DOI: 10.1016/j.cell.2016.11.053. View

Sternberg S, Doudna J . Expanding the Biologist's Toolkit with CRISPR-Cas9. Mol Cell. 2015; 58(4):568-74. DOI: 10.1016/j.molcel.2015.02.032. View

Abudayyeh O, Gootenberg J, Konermann S, Joung J, Slaymaker I, Cox D . C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016; 353(6299):aaf5573. PMC: 5127784. DOI: 10.1126/science.aaf5573. View

Yamano T, Nishimasu H, Zetsche B, Hirano H, Slaymaker I, Li Y . Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA. Cell. 2016; 165(4):949-62. PMC: 4899970. DOI: 10.1016/j.cell.2016.04.003. View

Jiang F, Zhou K, Ma L, Gressel S, Doudna J . STRUCTURAL BIOLOGY. A Cas9-guide RNA complex preorganized for target DNA recognition. Science. 2015; 348(6242):1477-81. DOI: 10.1126/science.aab1452. View

Anders C, Niewoehner O, Duerst A, Jinek M . Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease. Nature. 2014; 513(7519):569-73. PMC: 4176945. DOI: 10.1038/nature13579. View

10.

Marraffini L . CRISPR-Cas immunity in prokaryotes. Nature. 2015; 526(7571):55-61. DOI: 10.1038/nature15386. View

11.

Wright A, Nunez J, Doudna J . Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering. Cell. 2016; 164(1-2):29-44. DOI: 10.1016/j.cell.2015.12.035. View

12.

Liu L, Chen P, Wang M, Li X, Wang J, Yin M . C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism. Mol Cell. 2016; 65(2):310-322. DOI: 10.1016/j.molcel.2016.11.040. View

13.

Zetsche B, Gootenberg J, Abudayyeh O, Slaymaker I, Makarova K, Essletzbichler P . Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell. 2015; 163(3):759-71. PMC: 4638220. DOI: 10.1016/j.cell.2015.09.038. View

14.

Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna J, Charpentier E . A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 2012; 337(6096):816-21. PMC: 6286148. DOI: 10.1126/science.1225829. View