Lipid Quantification by Raman Microspectroscopy As a Potential Biomarker in Prostate Cancer

Overview

Journal Cancer Lett

Specialty Oncology

Date 2017 Mar 27

PMID 28342983

Citations 26

Authors

Jordan OMalley

Rahul Kumar

Andrey N Kuzmin

Artem Pliss

Neelu Yadav

Srimmitha Balachandar

Jianmin Wang

Kristopher Attwood

Paras N Prasad

Dhyan Chandra

Affiliations

Soon will be listed here.

Abstract

Metastatic castration-resistant prostate cancer (mCRPC) remains incurable and is one of the leading causes of cancer-related death among American men. Therefore, detection of prostate cancer (PCa) at early stages may reduce PCa-related mortality in men. We show that lipid quantification by vibrational Raman Microspectroscopy and Biomolecular Component Analysis may serve as a potential biomarker in PCa. Transcript levels of lipogenic genes including sterol regulatory element-binding protein-1 (SREBP-1) and its downstream effector fatty acid synthase (FASN), and rate-limiting enzyme acetyl CoA carboxylase (ACACA) were upregulated corresponding to both Gleason score and pathologic T stage in the PRAD TCGA cohort. Increased lipid accumulation in late-stage transgenic adenocarcinoma of mouse prostate (TRAMP) tumors compared to early-stage TRAMP and normal prostate tissues were observed. FASN along with other lipogenesis enzymes, and SREBP-1 proteins were upregulated in TRAMP tumors compared to wild-type prostatic tissues. Genetic alterations of key lipogenic genes predicted the overall patient survival using TCGA PRAD cohort. Correlation between lipid accumulation and tumor stage provides quantitative marker for PCa diagnosis. Thus, Raman spectroscopy-based lipid quantification could be a sensitive and reliable tool for PCa diagnosis and staging.

Citing Articles

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References

Svensson R, Parker S, Eichner L, Kolar M, Wallace M, Brun S . Inhibition of acetyl-CoA carboxylase suppresses fatty acid synthesis and tumor growth of non-small-cell lung cancer in preclinical models. Nat Med. 2016; 22(10):1108-1119. PMC: 5053891. DOI: 10.1038/nm.4181. View

Gutierrez-Pajares J, Hassen C, Chevalier S, Frank P . SR-BI: Linking Cholesterol and Lipoprotein Metabolism with Breast and Prostate Cancer. Front Pharmacol. 2016; 7:338. PMC: 5054001. DOI: 10.3389/fphar.2016.00338. View

Wang X, Sato R, Brown M, Hua X, Goldstein J . SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis. Cell. 1994; 77(1):53-62. DOI: 10.1016/0092-8674(94)90234-8. View

Ettinger S, Sobel R, Whitmore T, Akbari M, Bradley D, Gleave M . Dysregulation of sterol response element-binding proteins and downstream effectors in prostate cancer during progression to androgen independence. Cancer Res. 2004; 64(6):2212-21. DOI: 10.1158/0008-5472.can-2148-2. View

Leon C, Locke J, Adomat H, Etinger S, Twiddy A, Neumann R . Alterations in cholesterol regulation contribute to the production of intratumoral androgens during progression to castration-resistant prostate cancer in a mouse xenograft model. Prostate. 2009; 70(4):390-400. DOI: 10.1002/pros.21072. View

Liu Y . Fatty acid oxidation is a dominant bioenergetic pathway in prostate cancer. Prostate Cancer Prostatic Dis. 2006; 9(3):230-4. DOI: 10.1038/sj.pcan.4500879. View

Schlaepfer I, Nambiar D, Ramteke A, Kumar R, Dhar D, Agarwal C . Hypoxia induces triglycerides accumulation in prostate cancer cells and extracellular vesicles supporting growth and invasiveness following reoxygenation. Oncotarget. 2015; 6(26):22836-56. PMC: 4673203. DOI: 10.18632/oncotarget.4479. View

Yadav N, Pliss A, Kuzmin A, Rapali P, Sun L, Prasad P . Transformations of the macromolecular landscape at mitochondria during DNA-damage-induced apoptotic cell death. Cell Death Dis. 2014; 5:e1453. PMC: 4649512. DOI: 10.1038/cddis.2014.405. View

Gao S, Ye H, Gerrin S, Wang H, Sharma A, Chen S . ErbB2 Signaling Increases Androgen Receptor Expression in Abiraterone-Resistant Prostate Cancer. Clin Cancer Res. 2016; 22(14):3672-82. PMC: 4947432. DOI: 10.1158/1078-0432.CCR-15-2309. View

10.

Ding C, Yu W, Feng J, Luo J . Structure and function of Gab2 and its role in cancer (Review). Mol Med Rep. 2015; 12(3):4007-4014. PMC: 4526075. DOI: 10.3892/mmr.2015.3951. View

11.

Chang K, Li R, Papari-Zareei M, Watumull L, Zhao Y, Auchus R . Dihydrotestosterone synthesis bypasses testosterone to drive castration-resistant prostate cancer. Proc Natl Acad Sci U S A. 2011; 108(33):13728-33. PMC: 3158152. DOI: 10.1073/pnas.1107898108. View

12.

Spratt D, Evans M, Davis B, Doran M, Lee M, Shah N . Androgen Receptor Upregulation Mediates Radioresistance after Ionizing Radiation. Cancer Res. 2015; 75(22):4688-96. PMC: 4651750. DOI: 10.1158/0008-5472.CAN-15-0892. View

13.

Rae C, Haberkorn U, Babich J, Mairs R . Inhibition of Fatty Acid Synthase Sensitizes Prostate Cancer Cells to Radiotherapy. Radiat Res. 2015; 184(5):482-93. DOI: 10.1667/RR14173.1. View

14.

Zhang W, Liu H . MAPK signal pathways in the regulation of cell proliferation in mammalian cells. Cell Res. 2002; 12(1):9-18. DOI: 10.1038/sj.cr.7290105. View

15.

Sharifi N, Auchus R . Steroid biosynthesis and prostate cancer. Steroids. 2012; 77(7):719-26. DOI: 10.1016/j.steroids.2012.03.015. View

16.

Nambiar D, Deep G, Singh R, Agarwal C, Agarwal R . Silibinin inhibits aberrant lipid metabolism, proliferation and emergence of androgen-independence in prostate cancer cells via primarily targeting the sterol response element binding protein 1. Oncotarget. 2014; 5(20):10017-33. PMC: 4259402. DOI: 10.18632/oncotarget.2488. View

17.

Kast R, Tucker S, Killian K, Trexler M, Honn K, Auner G . Emerging technology: applications of Raman spectroscopy for prostate cancer. Cancer Metastasis Rev. 2014; 33(2-3):673-93. DOI: 10.1007/s10555-013-9489-6. View

18.

Chandra D, Liu J, Tang D . Early mitochondrial activation and cytochrome c up-regulation during apoptosis. J Biol Chem. 2002; 277(52):50842-54. DOI: 10.1074/jbc.M207622200. View

19.

Ventura R, Mordec K, Waszczuk J, Wang Z, Lai J, Fridlib M . Inhibition of de novo Palmitate Synthesis by Fatty Acid Synthase Induces Apoptosis in Tumor Cells by Remodeling Cell Membranes, Inhibiting Signaling Pathways, and Reprogramming Gene Expression. EBioMedicine. 2015; 2(8):808-24. PMC: 4563160. DOI: 10.1016/j.ebiom.2015.06.020. View

20.

Pliss A, Kuzmin A, Kachynski A, Prasad P . Biophotonic probing of macromolecular transformations during apoptosis. Proc Natl Acad Sci U S A. 2010; 107(29):12771-6. PMC: 2919951. DOI: 10.1073/pnas.1006374107. View