Hugonella Massiliensis Gen. Nov., Sp. Nov., Genome Sequence, and Description of a New Strictly Anaerobic Bacterium Isolated from the Human Gut

Overview

Journal Microbiologyopen

Specialty Microbiology

Date 2017 Mar 23

PMID 28326685

Citations 11

Authors

Ziena Elsawi

Amadou Hamidou Togo

Mamadou Beye

Gregory Dubourg

Claudia Andrieu

Nicholas Armsrtong

Magali Richez

Fabrizio Di Pinto

Fadi Bittar

Noemie Labas

Pierre-Edouard Fournier

Didier Raoult

Saber Khelaifia

Affiliations

Soon will be listed here.

Abstract

The human gut is composed of a large diversity of microorganisms, which have been poorly described. Here, using culturomics, a new concept based on the variation in culture conditions and MALDI-TOF MS identification, we proceed to explore the microbial diversity of the complex ecosystem of the human gut. Using this approach, we isolated strain AT8 (=CSUR P2118 = DSM 101782) from stool specimens collected from a 51-year-old obese French woman. Strain AT8 is a strictly anaerobic, nonmotile, nonspore-forming gram-positive coccus that do not exhibit catalase and oxidase activities. 16S rDNA-based identification of strain AT8 demonstrated 92% gene sequence similarity with Eggerthella lenta DSM 2243, the phylogenetically closed validly named type species. Here, we present a set of features for the strain AT8 and the description of its complete genome sequence and annotation. The 2,091,845 bp long genome has a G+C content of 63.46% and encodes1,849 predicted genes; 1,781 were protein-coding genes, and 68 were RNAs. On the basis of the characteristics reported here, we propose the creation of a new bacterial genus Hugonella gen. nov., belonging to the Eggerthellaceae family and including Hugonella massiliensis gen. nov., sp. nov., strain AT8 as the type strain.

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