Griottes: a Generalist Tool for Network Generation from Segmented Tissue Images

Overview

Journal BMC Biol

Publisher Biomed Central

Specialty Biology

Date 2022 Aug 11

PMID 35953853

Authors

Gustave Ronteix

Andrey Aristov

Valentin Bonnet

Sebastien Sart

Jeremie Sobel

Elric Esposito

Charles N Baroud

Affiliations

Soon will be listed here.

Abstract

Background: Microscopy techniques and image segmentation algorithms have improved dramatically this decade, leading to an ever increasing amount of biological images and a greater reliance on imaging to investigate biological questions. This has created a need for methods to extract the relevant information on the behaviors of cells and their interactions, while reducing the amount of computing power required to organize this information.

Results: This task can be performed by using a network representation in which the cells and their properties are encoded in the nodes, while the neighborhood interactions are encoded by the links. Here, we introduce Griottes, an open-source tool to build the "network twin" of 2D and 3D tissues from segmented microscopy images. We show how the library can provide a wide range of biologically relevant metrics on individual cells and their neighborhoods, with the objective of providing multi-scale biological insights. The library's capacities are demonstrated on different image and data types.

Conclusions: This library is provided as an open-source tool that can be integrated into common image analysis workflows to increase their capacities.

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