Time-dependent AluI Action on Human Chromosomes

Overview

Journal Genetica

Specialties Cell Biology
Genetics

Date 1991 Jan 1

PMID 1769563

Citations 1

Authors

A M Fernandez-Peralta

I Tagarro

P Ludena Reyes

C Sentis Castano

J Fernandez Piqueras

J J Gonzalez-Aguilera

Affiliations

Soon will be listed here.

Abstract

We have analyzed the pattern of AluI digestion over time on human chromosomes in order to monitor the evolution of the in situ enzyme action. Short treatments followed by Giemsa staining produce a G-like banding effect, whereas longer treatments produce a C-like banding pattern. However, when Propidium iodide staining is used, it reveals a uniform bright fluorescence after short AluI digestions and C bands when longer treatments are developed. We propose that C banding is the result of a uniform DNA removal in non centromeric regions taking place after a critical time point, the initial G like banding being produced by changes in the DNA-proteins interactions.

Citing Articles

Restriction endonuclease/nick translation procedure on fixed chromosomes of the Atlantic salmon fish cell line.

Abuin M, Martinez P, Sanchez L Chromosome Res. 1995; 3(6):379-85.

PMID: 7551554 DOI: 10.1007/BF00710020.

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