Interaction of Bacteriophage Lambda Repressor with Nonoperator DNA Containing Single-strand Gaps

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1978 Dec 1

PMID 282604

Citations 16

Authors

R Sussman

J Resnick

K Calame

J Baluch

Affiliations

Soon will be listed here.

Abstract

In direct binding assays, purified lambdaind+ repressor displayed high affinity for nonoperator DNA containing single-strand gaps. Its affinity for this same DNA but completely double-stranded, nicked, or denatured was considerably lower. In contrast, purified lambdaind- repressor had 1/10th the affinity for the gapped DNA, a level comparable to that of purified lac repressor. In the presence of limiting amounts of ind+ repressor, nonoperator DNA containing gaps could be shown to compete effectively with lambda DNA for binding of repressor. A previous model of lambda induction [Sussman, R. & Ben-Zeev, H. (1975) Proc. Natl. Acad. Sci. USA 72, 1973--1976], based on the assumption that this phenomenon involves the binding of repressor to lesions in the host DNA, is reevaluated in the light of the data reported here.

Citing Articles

Serendipity.

Sussman R J Biol Chem. 2008; 284(16):10285-90.

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Ultraviolet light induction of recA protein in a recB uvrB mutant of Escherichia coli.

Bockrath R, HANAWALT P J Bacteriol. 1980; 143(2):1025-8.

PMID: 7009541 PMC: 294408. DOI: 10.1128/jb.143.2.1025-1028.1980.

Restriction in vivo. V. Introduction of SOS functions in Escherichia coli by restricted T4 phage DNA, and alleviation of restriction by SOS functions.

Dharmalingam K, GOLDBERG E Mol Gen Genet. 1980; 178(1):51-8.

PMID: 6991879 DOI: 10.1007/BF00267212.

Effect of ssbA1 and lexC113 mutations on lambda prophage induction, bacteriophage growth, and cell survival.

Vales L, Chase J, Murphy J J Bacteriol. 1980; 143(2):887-96.

PMID: 6451615 PMC: 294387. DOI: 10.1128/jb.143.2.887-896.1980.

Synthesis of recA protein and induction of bacteriophage lambda in single-strand deoxyribonucleic acid-binding protein mutants of Escherichia coli.

Baluch J, Chase J, Sussman R J Bacteriol. 1980; 144(2):489-98.

PMID: 6448838 PMC: 294695. DOI: 10.1128/jb.144.2.489-498.1980.

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