Systems Imaging of the Immune Synapse

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2017 Mar 4

PMID 28255716

Citations 5

Authors

Rachel Ambler

Xiangtao Ruan

Robert F Murphy

Christoph Wulfing

Affiliations

Soon will be listed here.

Abstract

Three-dimensional live cell imaging of the interaction of T cells with antigen-presenting cells (APCs) visualizes the subcellular distributions of signaling intermediates during T cell activation at thousands of resolved positions within a cell. These information-rich maps of local protein concentrations are a valuable resource in understanding T cell signaling. Here, we describe a protocol for the efficient acquisition of such imaging data and their computational processing to create four-dimensional maps of local concentrations. This protocol allows quantitative analysis of T cell signaling as it occurs inside live cells with resolution in time and space across thousands of cells.

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