Detection of Hepatitis A Virus by Extraction of Viral RNA and Molecular Hybridization

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1987 Oct 1

PMID 2822759

Citations 9

Authors

J R Ticehurst

S M Feinstone

T Chestnut

N C Tassopoulos

H Popper

R H Purcell

Affiliations

Soon will be listed here.

Abstract

Hepatitis A virus (HAV) RNA was extracted from cell culture, serum, liver, and feces and then detected by molecular hybridization with cloned HAV cDNA. Hybridization was approximately 10-fold more sensitive than immune electron microscopy or radioimmunoassay was and less sensitive than was assays of HAV infectivity in primates or in cell culture. As little as 10(3) 50% infective doses of HAV, or approximately 0.1 pg of viral RNA, was detected by this method. Analysis of fecal specimens from an experimentally infected marmoset and an epidemic of hepatitis A showed that HAV excretion could often be detected later in the illness by hybridization than by radioimmunoassay. This technique should be widely applicable for detection and analysis of HAV RNA.

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