A Dot Hybridisation Assay for Detection of Rotavirus

Overview

Journal Lancet

Publisher Elsevier

Specialty General Medicine

Date 1983 Mar 12

PMID 6131257

Citations 26

Authors

J Flores

E Boeggeman

R H Purcell

M Sereno

I Perez

L White

R G Wyatt

R M Chanock

A Z Kapikian

Affiliations

Soon will be listed here.

Abstract

A "dot" hybridisation technique for the detection of rotavirus in stools and other biological materials is described. The assay is based on the in-situ hybridisation of labelled single-stranded RNA probes, obtained by in-vitro transcription of rotavirus particles, to heat-denatured rotavirus RNA immobilised on nitrocellulose membranes. The method is highly specific and allows for the detection of as little as 8 pg of viral RNA. Its use for the detection of rotavirus in stool suspensions and rectal swabs obtained from children with diarrhoea may facilitate epidemiological studies of rotavirus gastroenteritis.

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