Characterizing IsomiR Variants Within the MicroRNA-34/449 Family

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2017 Feb 14

PMID 28192603

Citations 34

Authors

Olivier Mercey

Alexandra Popa

Amelie Cavard

Agnes Paquet

Benoit Chevalier

Nicolas Pons

Virginie Magnone

Josephine Zangari

Patrick Brest

Laure-Emmanuelle Zaragosi

Gilles Ponzio

Kevin Lebrigand

Pascal Barbry

Brice Marcet

Affiliations

Soon will be listed here.

Abstract

miR-34/449 microRNAs are conserved regulators of multiciliated cell differentiation. Here, we evidence and characterize expression of two isomiR variant sequences from the miR-34/449 family in human airway epithelial cells. These isomiRs differ from their canonical counterparts miR-34b and miR-449c by one supplemental uridine at their 5'-end, leading to a one-base shift in their seed region. Overexpression of canonical miR-34/449 or 5'-isomiR-34/449 induces distinct gene expression profiles and biological effects. However, some target transcripts and functional activities are shared by both canonical microRNAs and isomiRs. Indeed, both repress important targets that result in cell cycle blockage and Notch pathway inhibition. Our findings suggest that 5'-isomiR-34/449 may represent additional mechanisms by which miR-34/449 family finely controls several pathways to drive multiciliogenesis.

Citing Articles

5'‑isomiR is the most abundant sequence of miR‑1246, a candidate biomarker of lung cancer, in serum.

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One locus, several functional RNAs-emerging roles of the mechanisms responsible for the sequence variability of microRNAs.

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A comprehensive pan-cancer analysis reveals cancer-associated robust isomiR expression landscapes in miRNA arm switching.

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The Dynamics of miR-449a/c Expression during Uterine Cycles Are Associated with Endometrial Development.

Naydenov M, Nikolova M, Apostolov A, Glogovitis I, Salumets A, Baev V Biology (Basel). 2023; 12(1).

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A novel approach for a joint analysis of isomiR and mRNA expression data reveals features of isomiR targeting in breast cancer.

Nersisyan S, Zhiyanov A, Engibaryan N, Maltseva D, Tonevitsky A Front Genet. 2022; 13:1070528.

PMID: 36531236 PMC: 9751988. DOI: 10.3389/fgene.2022.1070528.

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