Two MiRNA Clusters, MiR-34b/c and MiR-449, Are Essential for Normal Brain Development, Motile Ciliogenesis, and Spermatogenesis

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2014 Jul 2

PMID 24982181

Citations 155

Authors

Jingwen Wu

Jianqiang Bao

Minkyung Kim

Shuiqiao Yuan

Chong Tang

Huili Zheng

Grant S Mastick

Chen Xu

Wei Yan

Affiliations

Soon will be listed here.

Abstract

Ablation of a single miRNA gene rarely leads to a discernable developmental phenotype in mice, in some cases because of compensatory effects by other functionally related miRNAs. Here, we report that simultaneous inactivation of two functionally related miRNA clusters (miR-34b/c and miR-449) encoding five miRNAs (miR-34b, miR-34c, miR-449a, miR-449b, and miR-449c) led to sexually dimorphic, partial perinatal lethality, growth retardation, and infertility. These developmental defects correlated with the dysregulation of ∼ 240 target genes, which are mainly involved in three major cellular functions, including cell-fate control, brain development and microtubule dynamics. Our data demonstrate an essential role of a miRNA family in brain development, motile ciliogenesis, and spermatogenesis.

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