Chromosomal Integration of the Klebsiella Pneumoniae Carbapenemase Gene, , in Klebsiella Species Is Elusive but Not Rare

Carbapenemase genes in are mostly described as being plasmid associated. However, the genetic context of carbapenemase genes is not always confirmed in epidemiological surveys, and the frequency of their chromosomal integration therefore is unknown. A previously sequenced collection of -positive from a single U.S. institution (2007 to 2012; = 281 isolates from 182 patients) was analyzed to identify chromosomal insertions of Tn, the transposon most frequently harboring Using a combination of short- and long-read sequencing, we confirmed five independent chromosomal integration events from 6/182 (3%) patients, corresponding to 15/281 (5%) isolates. Three patients had isolates identified by perirectal screening, and three had infections which were all successfully treated. When a single copy of was in the chromosome, one or both of the phenotypic carbapenemase tests were negative. All chromosomally integrated genes were from spp., predominantly clonal group 258 (CG258), even though these represented only a small proportion of the isolates. Integration occurred via IS-ΔI-mediated transposition of a larger, composite region encompassing Tn at one locus of chromosomal integration, seen in the same strain ( ST340) in two patients. In summary, we identified five independent chromosomal integrations of in a large outbreak, demonstrating that this is not a rare event. was more frequently integrated into the chromosome of epidemic CG258 lineages (ST11, ST258, and ST340) and was more difficult to detect by routine phenotypic methods in this context. The presence of chromosomally integrated within successful, globally disseminated strains therefore is likely underestimated.