Proteomics Reveals the Effects of Sustained Weight Loss on the Human Plasma Proteome

Overview

Journal Mol Syst Biol

Specialty Molecular Biology

Date 2016 Dec 24

PMID 28007936

Citations 112

Authors

Philipp E Geyer

Nicolai J Wewer Albrechtsen

Stefka Tyanova

Niklas Grassl

Eva W Iepsen

Julie Lundgren

Sten Madsbad

Jens J Holst

Signe S Torekov

Matthias Mann

Affiliations

Soon will be listed here.

Abstract

Sustained weight loss is a preferred intervention in a wide range of metabolic conditions, but the effects on an individual's health state remain ill-defined. Here, we investigate the plasma proteomes of a cohort of 43 obese individuals that had undergone 8 weeks of 12% body weight loss followed by a year of weight maintenance. Using mass spectrometry-based plasma proteome profiling, we measured 1,294 plasma proteomes. Longitudinal monitoring of the cohort revealed individual-specific protein levels with wide-ranging effects of losing weight on the plasma proteome reflected in 93 significantly affected proteins. The adipocyte-secreted SERPINF1 and apolipoprotein APOF1 were most significantly regulated with fold changes of -16% and +37%, respectively (P < 10), and the entire apolipoprotein family showed characteristic differential regulation. Clinical laboratory parameters are reflected in the plasma proteome, and eight plasma proteins correlated better with insulin resistance than the known marker adiponectin. Nearly all study participants benefited from weight loss regarding a ten-protein inflammation panel defined from the proteomics data. We conclude that plasma proteome profiling broadly evaluates and monitors intervention in metabolic diseases.

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