Protein Structural Analysis Via Mass Spectrometry-Based Proteomics

Overview

Journal Adv Exp Med Biol

Specialties Biology
General Medicine

Date 2016 Dec 16

PMID 27975228

Citations 11

Authors

Antonio Artigues

Owen W Nadeau

Mary Ashley Rimmer

Maria T Villar

Xiuxia Du

Aron W Fenton

Gerald M Carlson

Affiliations

Soon will be listed here.

Abstract

Modern mass spectrometry (MS) technologies have provided a versatile platform that can be combined with a large number of techniques to analyze protein structure and dynamics. These techniques include the three detailed in this chapter: (1) hydrogen/deuterium exchange (HDX), (2) limited proteolysis, and (3) chemical crosslinking (CX). HDX relies on the change in mass of a protein upon its dilution into deuterated buffer, which results in varied deuterium content within its backbone amides. Structural information on surface exposed, flexible or disordered linker regions of proteins can be achieved through limited proteolysis, using a variety of proteases and only small extents of digestion. CX refers to the covalent coupling of distinct chemical species and has been used to analyze the structure, function and interactions of proteins by identifying crosslinking sites that are formed by small multi-functional reagents, termed crosslinkers. Each of these MS applications is capable of revealing structural information for proteins when used either with or without other typical high resolution techniques, including NMR and X-ray crystallography.

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