Self-organized Amniogenesis by Human Pluripotent Stem Cells in a Biomimetic Implantation-like Niche

Overview

Journal Nat Mater

Date 2016 Dec 13

PMID 27941807

Citations 106

Authors

Yue Shao

Kenichiro Taniguchi

Katherine Gurdziel

Ryan F Townshend

Xufeng Xue

Koh Meng Aw Yong

Jianming Sang

Jason R Spence

Deborah L Gumucio

Jianping Fu

Affiliations

Soon will be listed here.

Abstract

Amniogenesis-the development of amnion-is a critical developmental milestone for early human embryogenesis and successful pregnancy. However, human amniogenesis is poorly understood due to limited accessibility to peri-implantation embryos and a lack of in vitro models. Here we report an efficient biomaterial system to generate human amnion-like tissue in vitro through self-organized development of human pluripotent stem cells (hPSCs) in a bioengineered niche mimicking the in vivo implantation environment. We show that biophysical niche factors act as a switch to toggle hPSC self-renewal versus amniogenesis under self-renewal-permissive biochemical conditions. We identify a unique molecular signature of hPSC-derived amnion-like cells and show that endogenously activated BMP-SMAD signalling is required for the amnion-like tissue development by hPSCs. This study unveils the self-organizing and mechanosensitive nature of human amniogenesis and establishes the first hPSC-based model for investigating peri-implantation human amnion development, thereby helping advance human embryology and reproductive medicine.

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