To CRISPR and Beyond: the Evolution of Genome Editing in Stem Cells

Overview

Journal Regen Med

Specialty Biotechnology

Date 2016 Dec 2

PMID 27905217

Citations 3

Authors

Kuang-Yui Chen

Paul S Knoepfler

Affiliations

Soon will be listed here.

Abstract

The goal of editing the genomes of stem cells to generate model organisms and cell lines for genetic and biological studies has been pursued for decades. There is also exciting potential for future clinical impact in humans. While recent, rapid advances in targeted nuclease technologies have led to unprecedented accessibility and ease of gene editing, biology has benefited from past directed gene modification via homologous recombination, gene traps and other transgenic methodologies. Here we review the history of genome editing in stem cells (including via zinc finger nucleases, transcription activator-like effector nucleases and CRISPR-Cas9), discuss recent developments leading to the implementation of stem cell gene therapies in clinical trials and consider the prospects for future advances in this rapidly evolving field.

Citing Articles

Mechanisms regulating the CRISPR-Cas systems.

Zakrzewska M, Burmistrz M Front Microbiol. 2023; 14:1060337.

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FuncPEP: A Database of Functional Peptides Encoded by Non-Coding RNAs.

Dragomir M, Manyam G, Ott L, Berland L, Knutsen E, Ivan C Noncoding RNA. 2020; 6(4).

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Enhancing the Therapeutic Potential of Mesenchymal Stem Cells with the CRISPR-Cas System.

Filho D, de Carvalho Ribeiro P, Oliveira L, Terra Dos Santos A, Parreira R, Cunha Xavier Pinto M Stem Cell Rev Rep. 2019; 15(4):463-473.

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