Content of Total Phenolics, Flavan-3-Ols and Proanthocyanidins, Oxidative Stability and Antioxidant Capacity of Chocolate During Storage

Overview

Journal Food Technol Biotechnol

Specialty Biotechnology

Date 2016 Dec 2

PMID 27904388

Citations 7

Authors

Jovanka Lalicic-Petronijevic

Drazenka Komes

Stanislava Gorjanovic

Ana Belscak-Cvitanovic

Lato Pezo

Ferenc Pastor

Sanja Ostojic

Jovanka Popov-Raljic

Desanka Suznjevic

Affiliations

Soon will be listed here.

Abstract

Antioxidant (AO) capacity of chocolates with 27, 44 and 75% cocoa was assessed after production and during twelve months of storage by direct current (DC) polarographic assay, based on the decrease of anodic current caused by the formation of hydroxo-perhydroxyl mercury(II) complex (HPMC) in alkaline solutions of hydrogen peroxide at potentials of mercury oxidation, and two spectrophotometric assays. Relative antioxidant capacity index (RACI) was calculated by taking the average value of the AO assay (the sample mass in all assays was identical). Oxidative stability of chocolate fat was determined by differential scanning calorimetry. Measured parameters and RACI were correlated mutually and with the content of total phenols (Folin-Ciocalteu assay), flavan-3-ols (vanillin and -dimethylaminocinnamaldehyde assay) and proanthocyanidins (modified Bate-Smith assay). During storage, the studied functional and health-related characteristics remained unchanged. Amongst applied AO assays, the DC polarographic one, whose validity was confirmed by two-way ANOVA and F-test, correlated most significantly with oxidative stability (oxidation onset temperature and induction time). In addition, principal component analysis was applied to characterise chocolate types.

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References

Latif R . Chocolate/cocoa and human health: a review. Neth J Med. 2013; 71(2):63-8. View

Vinson J, Proch J, Bose P, Muchler S, Taffera P, Shuta D . Chocolate is a powerful ex vivo and in vivo antioxidant, an antiatherosclerotic agent in an animal model, and a significant contributor to antioxidants in the European and American Diets. J Agric Food Chem. 2006; 54(21):8071-6. DOI: 10.1021/jf062175j. View

Khan N, Khymenets O, Urpi-Sarda M, Tulipani S, Garcia-Aloy M, Monagas M . Cocoa polyphenols and inflammatory markers of cardiovascular disease. Nutrients. 2014; 6(2):844-80. PMC: 3942736. DOI: 10.3390/nu6020844. View

dEl-Rei J, Cunha A, Burla A, Burla M, Oigman W, Neves M . Characterisation of hypertensive patients with improved endothelial function after dark chocolate consumption. Int J Hypertens. 2013; 2013:985087. PMC: 3603200. DOI: 10.1155/2013/985087. View

Gorjanovic S, Pastor F, Vasic R, Novakovic M, Simonovic M, Milic S . Electrochemical versus spectrophotometric assessment of antioxidant activity of hop (Humulus lupulus L.) products and individual compounds. J Agric Food Chem. 2013; 61(38):9089-96. DOI: 10.1021/jf401718z. View

Hammerstone J, Lazarus S, Mitchell A, RUCKER R, SCHMITZ H . Identification of procyanidins in cocoa (Theobroma cacao) and chocolate using high-performance liquid chromatography/mass spectrometry. J Agric Food Chem. 1999; 47(2):490-6. DOI: 10.1021/jf980760h. View

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M . Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999; 26(9-10):1231-7. DOI: 10.1016/s0891-5849(98)00315-3. View

Baiano A, Gambacorta G, Terracone C, Previtali M, Lamacchia C, La Notte E . Changes in phenolic content and antioxidant activity of Italian extra-virgin olive oils during storage. J Food Sci. 2009; 74(2):C177-83. DOI: 10.1111/j.1750-3841.2009.01072.x. View

Fukumoto L, Mazza G . Assessing antioxidant and prooxidant activities of phenolic compounds. J Agric Food Chem. 2000; 48(8):3597-604. DOI: 10.1021/jf000220w. View

10.

Adamson G, Lazarus S, Mitchell A, Prior R, Cao G, Jacobs P . HPLC method for the quantification of procyanidins in cocoa and chocolate samples and correlation to total antioxidant capacity. J Agric Food Chem. 1999; 47(10):4184-8. DOI: 10.1021/jf990317m. View

11.

Friedman M, Levin C, Lee S, Kozukue N . Stability of green tea catechins in commercial tea leaves during storage for 6 months. J Food Sci. 2009; 74(2):H47-51. DOI: 10.1111/j.1750-3841.2008.01033.x. View

12.

Vertuani S, Scalambra E, Vittorio T, Bino A, Malisardi G, Baldisserotto A . Evaluation of antiradical activity of different cocoa and chocolate products: relation with lipid and protein composition. J Med Food. 2014; 17(4):512-6. PMC: 3993070. DOI: 10.1089/jmf.2013.0110. View

13.

Fernandez-Murga L, Tarin J, Garcia-Perez M, Cano A . The impact of chocolate on cardiovascular health. Maturitas. 2011; 69(4):312-21. DOI: 10.1016/j.maturitas.2011.05.011. View

14.

Arranz S, Valderas-Martinez P, Chiva-Blanch G, Casas R, Urpi-Sarda M, Lamuela-Raventos R . Cardioprotective effects of cocoa: clinical evidence from randomized clinical intervention trials in humans. Mol Nutr Food Res. 2013; 57(6):936-47. DOI: 10.1002/mnfr.201200595. View

15.

Suznjevic D, Pastor F, Gorjanovic S . Polarographic study of hydrogen peroxide anodic current and its application to antioxidant activity determination. Talanta. 2011; 85(3):1398-403. DOI: 10.1016/j.talanta.2011.06.039. View

16.

Hurst W, Payne M, Miller K, Stuart D . Stability of cocoa antioxidants and flavan-3-ols over time. J Agric Food Chem. 2010; 57(20):9547-50. DOI: 10.1021/jf901457s. View

17.

Steinberg F, Bearden M, Keen C . Cocoa and chocolate flavonoids: implications for cardiovascular health. J Am Diet Assoc. 2003; 103(2):215-23. DOI: 10.1053/jada.2003.50028. View

18.

Rusconi M, Conti A . Theobroma cacao L., the Food of the Gods: a scientific approach beyond myths and claims. Pharmacol Res. 2009; 61(1):5-13. DOI: 10.1016/j.phrs.2009.08.008. View

19.

Benzie I, Strain J . The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1):70-6. DOI: 10.1006/abio.1996.0292. View

20.

Gorjanovic S, Komes D, Pastor F, Belscak-Cvitanovic A, Pezo L, Hecimovic I . Antioxidant capacity of teas and herbal infusions: polarographic assessment. J Agric Food Chem. 2012; 60(38):9573-80. DOI: 10.1021/jf302375t. View