Genetic Control of Eosinophilia. Analysis of Production and Response to Eosinophil-differentiating Factor in Strains of Mice Infected with Trichinella Spiralis

Bone marrow cultures were established from mice undergoing parasitic eosinophilia after infection with Trichinella spiralis. In the presence of eosinophil-differentiation factor (EDF/IL-5) eosinophil precursor cells differentiated and could be identified and counted after a 7-day in vitro culture period. The EDF-bone marrow assay system was used to determine differences in bone marrow eosinophil precursor capacity between a number of inbred strains of mice. Bone marrow cultures from high peripheral eosinophil-response phenotype strains of mice (NIH, SWR & SJL) contained significantly greater numbers of eosinophil precursor cells than the low response strain C57BL/10. All congenic strains of mice with the B10 background, i.e. C57BL/10, B10.S, B10.BR and B10.G were found to have low eosinophil precursor capacity. Bone marrow cultures obtained from F1 hybrids (NIH x C57/BL10, SJL x C57/BL10 and SWR x C57BL/10) demonstrated high precursor numbers, indicating that low responsiveness is inherited as a recessive characteristic. When spleen cells from T. spiralis-infected, high and low responder strains of mice were stimulated in vitro with concanavalin A (Con A) or with parasite antigen, it was found that low responder phenotype strains produced quantities of two eosinophilopoietic lymphokines EDF and IL3, which were similar to, if not greater than high responder strains. This suggests that bone marrow precursor capacity and not T cell lymphokine release is an important limiting factor in determining strain-dependent eosinophilia.