PRDM14 Drives OCT3/4 Recruitment Via Active Demethylation in the Transition from Primed to Naive Pluripotency

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2016 Nov 22

PMID 27866876

Citations 21

Authors

Naoki Okashita

Yoshiaki Suwa

Osamu Nishimura

Nao Sakashita

Mitsutaka Kadota

Go Nagamatsu

Masanori Kawaguchi

Hiroki Kashida

Ayaka Nakajima

Makoto Tachibana

Yoshiyuki Seki

Affiliations

Soon will be listed here.

Abstract

Primordial germ cells (PGCs) are specified from epiblast cells in mice. Genes associated with naive pluripotency are repressed in the transition from inner cell mass to epiblast cells, followed by upregulation after PGC specification. However, the molecular mechanisms underlying the reactivation of pluripotency genes are poorly characterized. Here, we exploited the in vitro differentiation of epiblast-like cells (EpiLCs) from embryonic stem cells (ESCs) to elucidate the molecular and epigenetic functions of PR domain-containing 14 (PRDM14). We found that Prdm14 overexpression in EpiLCs induced their conversion to ESC-like cells even in the absence of leukemia inhibitory factor in adherent culture. This was impaired by the loss of Kruppel-like factor 2 and ten-eleven translocation (TET) proteins. Furthermore, PRDM14 recruited OCT3/4 to the enhancer regions of naive pluripotency genes via TET-base excision repair-mediated demethylation. Our results provide evidence that PRDM14 establishes a transcriptional network for naive pluripotency via active DNA demethylation.

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