Mapping of Antibody Specificities to VH Gene Families

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1989 Jan 1

PMID 2785504

Citations 4

Authors

G Kelsoe

R Miceli

J cerny

D H Schulze

Affiliations

Soon will be listed here.

Abstract

VH gene segments represent the products of the repeated duplication and subsequent diversification of a primordial V gene element. It is widely assumed that natural selection, operating via pathogens, has played the dominant role in this process. Here, we screen some 3.7 x 10(4) C mu+ colonies of mitogen-activated B cells for the production of antibodies specific for phosphorylcholine or hen egg lysozyme and expression of the VH X-24, S107, Q52, or J558 gene families. These gene families were expressed at frequencies proportional to their genomic complexity among both unselected and antigen-specific C mu+ colonies. Thus, the capacity to encode equivalent antibody-combining sites is dispersed uniformly among VH families. This result suggests that individual VH genes have not evolved to address specific antigens.

Citing Articles

Evolution of V genes: DNA sequence structure of functional germline genes and pseudogenes.

Rothenfluh H, Blanden R, Steele E Immunogenetics. 1995; 42(3):159-71.

PMID: 7642227 DOI: 10.1007/BF00191221.

Characterization of a complete immunoglobulin heavy-chain variable region germ-line gene of rainbow trout.

Matsunaga T, Chen T, Tormanen V Proc Natl Acad Sci U S A. 1990; 87(19):7767-71.

PMID: 2120708 PMC: 54829. DOI: 10.1073/pnas.87.19.7767.

Stochastic pairing of heavy-chain and kappa light-chain variable gene families occurs in polyclonally activated B cells.

Kaushik A, Schulze D, Bonilla F, Bona C, Kelsoe G Proc Natl Acad Sci U S A. 1990; 87(13):4932-6.

PMID: 2114644 PMC: 54235. DOI: 10.1073/pnas.87.13.4932.

In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. I. The architecture and dynamics of responding cell populations.

Jacob J, Kassir R, Kelsoe G J Exp Med. 1991; 173(5):1165-75.

PMID: 1902502 PMC: 2118845. DOI: 10.1084/jem.173.5.1165.

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