A Novel Selective Medium for Isolation of Bacteroides Fragilis from Clinical Specimens

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2016 Nov 18

PMID 27852672

Citations 3

Authors

Pak-Leung Ho

Lok-Yan Ho

Chong-Yee Yau

Man-Ki Tong

Kin-Hung Chow

Affiliations

Soon will be listed here.

Abstract

A novel Bacteroides fragilis selective (BFS) medium, consisting of a brain heart infusion agar base supplemented with yeast extract, cysteine hydrochloride, bile salts, vitamin K, hemin, glucose, esculin, ferric ammonium citrate, bromothymol blue, gentamicin, kanamycin, and novobiocin, was evaluated. When BFS agar was tested with a collection of 303 bacteria of different genera, it allowed the growth of B. fragilis as large yellow colonies, with blackening of the medium after 48 h of anaerobic incubation, while the growth of most other anaerobes, facultative anaerobes, and aerobes was inhibited. In a prospective comparison of BFS agar with a routinely used medium (neomycin blood agar) in 1,209 clinical specimens, 60 B. fragilis bacteria were detected on BFS agar while 46 were detected on the routine agar (McNemar's test, P = 0.008). In conclusion, this novel medium may be added to improve the recovery of B. fragilis in clinical specimens and to facilitate surveillance of antimicrobial-resistant strains.

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