Differentiation of CfiA-negative and CfiA-positive Bacteroides Fragilis Isolates by Matrix-assisted Laser Desorption Ionization-time of Flight Mass Spectrometry

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2011 Feb 25

PMID 21346046

Citations 25

Authors

Ingrid Wybo

Annelies De Bel

Oriane Soetens

Fedoua Echahidi

Kristof Vandoorslaer

Marina Van Cauwenbergh

Denis Pierard

Affiliations

Soon will be listed here.

Abstract

Carbapenem resistance in Bacteroides fragilis is associated with cfiA-encoded class B metallo-beta-lactamase. cfiA-negative and cfiA-positive isolates belong to genotypically distinct groups. Of a total of 248 B. fragilis isolates included in this study, 214 were susceptible, 10 were intermediate, and 24 were resistant to meropenem. We show that matrix-assisted laser desorption ionization-time of flight mass spectrometry is able to differentiate between cfiA-negative and cfiA-positive isolates and predict carbapenem resistance in a routine laboratory setting.

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