Skin Vaccination Against Rotavirus Using Microneedles: Proof of Concept in Gnotobiotic Piglets

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Nov 9

PMID 27824918

Citations 21

Authors

Yuhuan Wang

Anastasia Vlasova

Daniel E Velasquez

Linda J Saif

Sukumar Kandasamy

Efrat Kochba

Yotam Levin

Baoming Jiang

Affiliations

Soon will be listed here.

Abstract

Live-attenuated oral rotavirus (RV) vaccines have lower efficacy in low income countries, and additionally are associated with a rare but severe adverse event, intussusception. We have been pursuing the development of an inactivated rotavirus vaccine (IRV) using the human rotavirus strain CDC-9 (G1P[8]) through parenteral immunization and previously demonstrated dose sparing and enhanced immunogenicity of intradermal (ID) unadjuvanted IRV using a coated microneedle patch in comparison with intramuscular (IM) administration in mice. The aim of this study was to evaluate the immune response and protection against RV infection and diarrhea conferred by the administration of the ID unadjuvanted IRV using the microneedle device MicronJet600® in neonatal gnotobiotic (Gn) piglets challenged with virulent Wa G1P[8] human RV. Three doses of 5 μg IRV when administered intradermally and 5 μg IRV formulated with aluminum hydroxide [Al(OH)3] when administered intramuscularly induced comparable rotavirus-specific antibody titers of IgA, IgG, IgG avidity index and neutralizing activity in sera of neonatal piglets. Both IRV vaccination regimens protected against RV antigen shedding in stools, and reduced the cumulative diarrhea scores in the piglets. This study demonstrated that the ID and IM administrations of IRV are immunogenic and protective against RV-induced diarrhea in neonatal piglets. Our findings highlight the potential value of an adjuvant sparing effect of the IRV ID delivery route.

Citing Articles

Correlates of immune protection against human rotaviruses: natural infection and vaccination.

Latifi T, Kachooei A, Jalilvand S, Zafarian S, Roohvand F, Shoja Z Arch Virol. 2024; 169(3):72.

PMID: 38459213 DOI: 10.1007/s00705-024-05975-y.

Skin-Based Vaccination: A Systematic Mapping Review of the Types of Vaccines and Methods Used and Immunity and Protection Elicited in Pigs.

Co-Rives I, Chen A, Moore A Vaccines (Basel). 2023; 11(2).

PMID: 36851328 PMC: 9962282. DOI: 10.3390/vaccines11020450.

Evaluation of efficacy and safety of intradermal delivery of vaccines through microneedle(s) in human beings: a protocol for a systematic review.

Bajwa I, Kaur N, Dsouza J, Mathew J Syst Rev. 2022; 11(1):170.

PMID: 35964062 PMC: 9375374. DOI: 10.1186/s13643-022-02046-8.

Microneedle patch as a new platform to effectively deliver inactivated polio vaccine and inactivated rotavirus vaccine.

Moon S, Richter-Roche M, Resch T, Wang Y, Foytich K, Wang H NPJ Vaccines. 2022; 7(1):26.

PMID: 35228554 PMC: 8885742. DOI: 10.1038/s41541-022-00443-7.

Rotavirus vaccines: progress and new developments.

Cates J, Tate J, Parashar U Expert Opin Biol Ther. 2021; 22(3):423-432.

PMID: 34482790 PMC: 10839819. DOI: 10.1080/14712598.2021.1977279.

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