Unlocking the Barley Genome by Chromosomal and Comparative Genomics

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 2011 Apr 7

PMID 21467582

Citations 211

Authors

Klaus F X Mayer

Mihaela Martis

Pete E Hedley

Hana Simkova

Hui Liu

Jenny A Morris

Burkhard Steuernagel

Stefan Taudien

Stephan Roessner

Heidrun Gundlach

Marie Kubalakova

Pavla Suchankova

Florent Murat

Marius Felder

Thomas Nussbaumer

Andreas Graner

Jerome Salse

Takashi Endo

Hiroaki Sakai

Tsuyoshi Tanaka

Takeshi Itoh

Kazuhiro Sato

Matthias Platzer

Takashi Matsumoto

Uwe Scholz

Jaroslav Dolezel

Robbie Waugh

Nils Stein

Affiliations

Soon will be listed here.

Abstract

We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign ~86% of the estimated ~32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.

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