Bright Photoactivatable Fluorophores for Single-molecule Imaging

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2016 Nov 8

PMID 27776112

Citations 165

Authors

Jonathan B Grimm

Brian P English

Heejun Choi

Anand K Muthusamy

Brian P Mehl

Peng Dong

Timothy A Brown

Jennifer Lippincott-Schwartz

Zhe Liu

Timothee Lionnet

Luke D Lavis

Affiliations

Soon will be listed here.

Abstract

Small-molecule fluorophores are important tools for advanced imaging experiments. We previously reported a general method to improve small, cell-permeable fluorophores which resulted in the azetidine-containing 'Janelia Fluor' (JF) dyes. Here, we refine and extend the utility of these dyes by synthesizing photoactivatable derivatives that are compatible with live-cell labeling strategies. Once activated, these derived compounds retain the superior brightness and photostability of the JF dyes, enabling improved single-particle tracking and facile localization microscopy experiments.

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