» Articles » PMID: 27748445

Dynamics of the Mouse Brain Cortical Synaptic Proteome During Postnatal Brain Development

Overview
Journal Sci Rep
Specialty Science
Date 2016 Oct 18
PMID 27748445
Citations 48
Authors
Affiliations
Soon will be listed here.
Abstract

Development of the brain involves the formation and maturation of numerous synapses. This process requires prominent changes of the synaptic proteome and potentially involves thousands of different proteins at every synapse. To date the proteome analysis of synapse development has been studied sparsely. Here, we analyzed the cortical synaptic membrane proteome of juvenile postnatal days 9 (P9), P15, P21, P27, adolescent (P35) and different adult ages P70, P140 and P280 of C57Bl6/J mice. Using a quantitative proteomics workflow we quantified 1560 proteins of which 696 showed statistically significant differences over time. Synaptic proteins generally showed increased levels during maturation, whereas proteins involved in protein synthesis generally decreased in abundance. In several cases, proteins from a single functional molecular entity, e.g., subunits of the NMDA receptor, showed differences in their temporal regulation, which may reflect specific synaptic development features of connectivity, strength and plasticity. SNARE proteins, Snap 29/47 and Stx 7/8/12, showed higher expression in immature animals. Finally, we evaluated the function of Cxadr that showed high expression levels at P9 and a fast decline in expression during neuronal development. Knock down of the expression of Cxadr in cultured primary mouse neurons revealed a significant decrease in synapse density.

Citing Articles

Proteomic Identification and Label-Free Quantification of Proteins Implicated in Neurite and Spine Formation.

Sharma K, Kaushal P, Kumar V Methods Mol Biol. 2024; 2831:133-143.

PMID: 39134848 DOI: 10.1007/978-1-0716-3969-6_10.


Remodeling of the postsynaptic proteome in male mice and marmosets during synapse development.

Kaizuka T, Suzuki T, Kishi N, Tamada K, Kilimann M, Ueyama T Nat Commun. 2024; 15(1):2496.

PMID: 38548776 PMC: 10979008. DOI: 10.1038/s41467-024-46529-9.


Identification of New Modulators and Inhibitors of Palmitoyl-Protein Thioesterase 1 for CLN1 Batten Disease and Cancer.

Puhl A, Raman R, Havener T, Minerali E, Hickey A, Ekins S ACS Omega. 2024; 9(10):11870-11882.

PMID: 38496939 PMC: 10938339. DOI: 10.1021/acsomega.3c09607.


Loss of the E3 ubiquitin ligase TRIM67 alters the post-synaptic density proteome.

McCormick L, Baker N, Herring L, Gupton S MicroPubl Biol. 2024; 2024.

PMID: 38495584 PMC: 10943362. DOI: 10.17912/micropub.biology.001118.


Nacc1 Mutation in Mice Models Rare Neurodevelopmental Disorder with Underlying Synaptic Dysfunction.

Deehan M, Kothuis J, Sapp E, Chase K, Ke Y, Seeley C J Neurosci. 2024; 44(14).

PMID: 38388424 PMC: 10993038. DOI: 10.1523/JNEUROSCI.1610-23.2024.


References
1.
Shilov I, Seymour S, Patel A, Loboda A, Tang W, Keating S . The Paragon Algorithm, a next generation search engine that uses sequence temperature values and feature probabilities to identify peptides from tandem mass spectra. Mol Cell Proteomics. 2007; 6(9):1638-55. DOI: 10.1074/mcp.T600050-MCP200. View

2.
Xu X, Deng C, Liu Y, He M, Peng J, Wang T . MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development. Cell Res. 2014; 24(5):576-94. PMC: 4011341. DOI: 10.1038/cr.2014.33. View

3.
Brauer A, Savaskan N, Kuhn H, Prehn S, Ninnemann O, Nitsch R . A new phospholipid phosphatase, PRG-1, is involved in axon growth and regenerative sprouting. Nat Neurosci. 2003; 6(6):572-8. DOI: 10.1038/nn1052. View

4.
Yang X, Hou D, Jiang W, Zhang C . Intercellular protein-protein interactions at synapses. Protein Cell. 2014; 5(6):420-44. PMC: 4026422. DOI: 10.1007/s13238-014-0054-z. View

5.
Chen N, Koopmans F, Gordon A, Paliukhovich I, Klaassen R, van der Schors R . Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes. Biochim Biophys Acta. 2015; 1854(7):827-33. DOI: 10.1016/j.bbapap.2015.02.008. View