Comparison of Serum Thiol-disulphide Homeostasis and Total Antioxidant-oxidant Levels Between Exudative Age-related Macular Degeneration Patients and Healthy Subjects

Overview

Journal Int Ophthalmol

Specialty Ophthalmology

Date 2016 Oct 14

PMID 27734243

Citations 3

Authors

Ahmet Elbay

Omer Faruk Ozer

Julide Canan Umurhan Akkan

Ugur Celik

Isil Kutluturk

Arif Koytak

Hakan Ozdemir

Affiliations

Soon will be listed here.

Abstract

Purpose: The purpose of the study was to calculate serum total oxidant status (TOS), total antioxidant status (TAS), and dynamic thiol-disulphide (T-D) homeostasis in patients with age-related macular degeneration (AMD), and compare the results with healthy individuals.

Methods: Thirty-three exudative AMD patients and 33 healthy controls were included in this case-control study. Participants' serum TAS and TOS levels were measured. In addition, total thiol (TT), native thiol (NT), and disulphide (DS) concentrations were assessed using a novel automated method of measurement.

Results: In comparison with the control group, serum TAS, TT, and NT levels were found to be significantly lower (p < 0.0001, p = 0.004, p = 0.003, respectively) and TOS levels were detected higher (p = 0.032) in AMD patients. Serum DS levels were elevated in the AMD patient group, but the difference was not statistically significant (p = 0.219). DS/TT and DS/NT ratios were significantly higher (p = 0.012, p = 0.013, respectively) in AMD patients. A positive correlation was found between TT and NT (p < 0.0001) in AMD group.

Conclusions: Serum TOS levels are higher, TAS levels are lower, and the T-D balance is shifted to the DS bond side in AMD patients. These results suggest that increased oxidative stress and decreased antioxidant levels may play a role in AMD progression. Further studies are needed to confirm the pathophysiologic role of T-D homeostasis in AMD.

Citing Articles

Carnosine supplementation and retinal oxidative parameters in a high-calorie diet rat model.

Torres R, Moreto F, Luchini A, Torres R, Longo S, Pinho R BMC Ophthalmol. 2023; 23(1):502.

PMID: 38066465 PMC: 10709828. DOI: 10.1186/s12886-023-03255-y.

Oxidative Stress and Antioxidants in Age-Related Macular Degeneration.

Kushwah N, Bora K, Maurya M, Pavlovich M, Chen J Antioxidants (Basel). 2023; 12(7).

PMID: 37507918 PMC: 10376043. DOI: 10.3390/antiox12071379.

Evaluation of oxidative stress, 3-Nitrotyrosine, and HMGB-1 levels in patients with wet type Age-Related Macular Degeneration.

Ramazan Z, Sari I, Yildirim B, Gunturk I, Kucuk E, Ersan S J Med Biochem. 2022; 41(3):275-281.

PMID: 36042902 PMC: 9375537. DOI: 10.5937/jomb0-32189.

References

Erel O, Neselioglu S . A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014; 47(18):326-32. DOI: 10.1016/j.clinbiochem.2014.09.026. View

Samiec P, Drews-Botsch C, Flagg E, Kurtz J, Sternberg Jr P, Reed R . Glutathione in human plasma: decline in association with aging, age-related macular degeneration, and diabetes. Free Radic Biol Med. 1998; 24(5):699-704. DOI: 10.1016/s0891-5849(97)00286-4. View

Plestina-Borjan I, Katusic D, Medvidovic-Grubisic M, Supe-Domic D, Bucan K, Tandara L . Association of age-related macular degeneration with erythrocyte antioxidant enzymes activity and serum total antioxidant status. Oxid Med Cell Longev. 2015; 2015:804054. PMC: 4359814. DOI: 10.1155/2015/804054. View

Jones D, Liang Y . Measuring the poise of thiol/disulfide couples in vivo. Free Radic Biol Med. 2009; 47(10):1329-38. PMC: 2774737. DOI: 10.1016/j.freeradbiomed.2009.08.021. View

Heiba I, Elston R, Klein B, Klein R . Sibling correlations and segregation analysis of age-related maculopathy: the Beaver Dam Eye Study. Genet Epidemiol. 1994; 11(1):51-67. DOI: 10.1002/gepi.1370110106. View

Erel O . A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004; 37(4):277-85. DOI: 10.1016/j.clinbiochem.2003.11.015. View

Erel O . A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005; 38(12):1103-11. DOI: 10.1016/j.clinbiochem.2005.08.008. View

Smith W, Mitchell P, Leeder S . Smoking and age-related maculopathy. The Blue Mountains Eye Study. Arch Ophthalmol. 1996; 114(12):1518-23. DOI: 10.1001/archopht.1996.01100140716016. View

Delcourt C, Cristol J, Leger C, Descomps B, Papoz L . Associations of antioxidant enzymes with cataract and age-related macular degeneration. The POLA Study. Pathologies Oculaires Liées à l'Age. Ophthalmology. 1999; 106(2):215-22. DOI: 10.1016/s0161-6420(99)90059-3. View

10.

Handa J, Verzijl N, Matsunaga H, Lutty G, te Koppele J, Miyata T . Increase in the advanced glycation end product pentosidine in Bruch's membrane with age. Invest Ophthalmol Vis Sci. 1999; 40(3):775-9. View

11.

Yildirim Z, Ucgun N, Yildirim F . The role of oxidative stress and antioxidants in the pathogenesis of age-related macular degeneration. Clinics (Sao Paulo). 2011; 66(5):743-6. PMC: 3109369. DOI: 10.1590/s1807-59322011000500006. View

12.

Handa J, Reiser K, Matsunaga H, Hjelmeland L . The advanced glycation endproduct pentosidine induces the expression of PDGF-B in human retinal pigment epithelial cells. Exp Eye Res. 1998; 66(4):411-9. DOI: 10.1006/exer.1997.0442. View

13.

Pauleikhoff D . neovascular age-related macular degeneration: Natural History and Treatment Outcomes. Retina. 2005; 25(8):1065-84. DOI: 10.1097/00006982-200512000-00016. View

14.

Evereklioglu C, Er H, Doganay S, Cekmen M, Turkoz Y, Otlu B . Nitric oxide and lipid peroxidation are increased and associated with decreased antioxidant enzyme activities in patients with age-related macular degeneration. Doc Ophthalmol. 2003; 106(2):129-36. DOI: 10.1023/a:1022512402811. View

15.

Smith W, Assink J, Klein R, Mitchell P, Klaver C, Klein B . Risk factors for age-related macular degeneration: Pooled findings from three continents. Ophthalmology. 2001; 108(4):697-704. DOI: 10.1016/s0161-6420(00)00580-7. View

16.

Seddon J, Cote J, Davis N, Rosner B . Progression of age-related macular degeneration: association with body mass index, waist circumference, and waist-hip ratio. Arch Ophthalmol. 2003; 121(6):785-92. DOI: 10.1001/archopht.121.6.785. View

17.

Ohia S, Opere C, Leday A . Pharmacological consequences of oxidative stress in ocular tissues. Mutat Res. 2005; 579(1-2):22-36. DOI: 10.1016/j.mrfmmm.2005.03.025. View

18.

. Krypton laser photocoagulation for neovascular lesions of age-related macular degeneration. Results of a randomized clinical trial. Macular Photocoagulation Study Group. Arch Ophthalmol. 1990; 108(6):816-24. DOI: 10.1001/archopht.1990.01070080058036. View

19.

Mitchell P, Wang J, Foran S, Smith W . Five-year incidence of age-related maculopathy lesions: the Blue Mountains Eye Study. Ophthalmology. 2002; 109(6):1092-7. DOI: 10.1016/s0161-6420(02)01055-2. View

20.

Brantley Jr M, Osborn M, Sanders B, Rezaei K, Lu P, Li C . Plasma biomarkers of oxidative stress and genetic variants in age-related macular degeneration. Am J Ophthalmol. 2011; 153(3):460-467.e1. PMC: 3288635. DOI: 10.1016/j.ajo.2011.08.033. View