Evaluation of Oxidative Stress, 3-Nitrotyrosine, and HMGB-1 Levels in Patients with Wet Type Age-Related Macular Degeneration

Overview

Journal J Med Biochem

Specialty Biochemistry

Date 2022 Aug 31

PMID 36042902

Authors

Zor Kursad Ramazan

Ismail Sari

Bicer Gamze Yildirim

Inayet Gunturk

Erkut Kucuk

Serpil Ersan

Gonul Seyda Seydel

Affiliations

Soon will be listed here.

Abstract

Background: This study aims to compare serum HMGB-1, 3-nitrotyrosine (3-NT), TAS, TOS, and OSI levels in Wettype Age-Related Macular Degeneration (wAMD) patients and healthy controls to determine the correlation of these parameters with each other.

Methods: Thirty patients with Wet-type Age-Related Macular Degeneration (wAMD) and 27 healthy adults, as controls were enrolled in the study. We determined the TAS and TOS levels in serum samples of both groups using commercial kits on a microplate reader. Serum HMGB-1 and 3-NT levels were measured with the enzyme-linked immunosorbent assay method.

Results: HMGB-1 levels were significantly higher in the patient group (137.51 pg/mL, p=0.001), while there was no difference between the two groups in serum 3-NT levels (p=0.428). A statistically significant difference found in the levels of TOS and OSI (p=0.001 and p=0.045, respectively) between the patients and controls, however, no significant difference was observed between the groups in terms of TAS levels (p=0.228).

Conclusions: Oxidative stress and HMGB-1 levels were increased in wAMD patients and enhanced oxidative stress may be associated with increased tissue necrosis and inflammation. Thus administration of antioxidant treatment in addition to routine therapy should be considered in wAMD.

Citing Articles

NMR Untargeted and HPLC-MS/MS Targeted Metabolomic Approaches for Evaluating Styrene Exposure in the Urine of Shipyard Workers.

Giampaoli O, Sciubba F, Tranfo G, Sisto R, Pigini D, De Rosa M Toxics. 2024; 12(3).

PMID: 38535915 PMC: 10975827. DOI: 10.3390/toxics12030182.

Oxidative Stress and Antioxidants in Age-Related Macular Degeneration.

Kushwah N, Bora K, Maurya M, Pavlovich M, Chen J Antioxidants (Basel). 2023; 12(7).

PMID: 37507918 PMC: 10376043. DOI: 10.3390/antiox12071379.

Oxidative stress-related risk of the multiple sclerosis development.

Vasic M, Topic A, Markovic B, Milinkovic N, Dincic E J Med Biochem. 2023; 42(1):1-8.

PMID: 36819128 PMC: 9920994. DOI: 10.5937/jomb0-37546.

References

van Beijnum J, Nowak-Sliwinska P, van den Boezem E, Hautvast P, Buurman W, Griffioen A . Tumor angiogenesis is enforced by autocrine regulation of high-mobility group box 1. Oncogene. 2012; 32(3):363-74. DOI: 10.1038/onc.2012.49. View

Altinisik M, Koytak A, Elbay A, Toklu E, Sezer T, Kocyigit A . Oxidant-Antioxidant Balance in the Aqueous Humor of Patients with Retinal Vein Occlusion. Semin Ophthalmol. 2017; 33(5):675-682. DOI: 10.1080/08820538.2017.1416412. View

Kikuchi H, Yagi H, Hasegawa H, Ishii Y, Okabayashi K, Tsuruta M . Therapeutic potential of transgenic mesenchymal stem cells engineered to mediate anti-high mobility group box 1 activity: targeting of colon cancer. J Surg Res. 2014; 190(1):134-43. DOI: 10.1016/j.jss.2014.02.047. View

Elbay A, Ozer O, Umurhan Akkan J, Celik U, Kutluturk I, Koytak A . Comparison of serum thiol-disulphide homeostasis and total antioxidant-oxidant levels between exudative age-related macular degeneration patients and healthy subjects. Int Ophthalmol. 2016; 37(5):1095-1101. DOI: 10.1007/s10792-016-0367-4. View

Hanus J, Anderson C, Wang S . RPE necroptosis in response to oxidative stress and in AMD. Ageing Res Rev. 2015; 24(Pt B):286-98. PMC: 4661094. DOI: 10.1016/j.arr.2015.09.002. View

Malarkey C, Churchill M . The high mobility group box: the ultimate utility player of a cell. Trends Biochem Sci. 2012; 37(12):553-62. PMC: 4437563. DOI: 10.1016/j.tibs.2012.09.003. View

Kauppinen A, Paterno J, Blasiak J, Salminen A, Kaarniranta K . Inflammation and its role in age-related macular degeneration. Cell Mol Life Sci. 2016; 73(9):1765-86. PMC: 4819943. DOI: 10.1007/s00018-016-2147-8. View

Wang B, Liu Y, Xu Q, Wang J . [Investigation to the levels of 3-nitrotyrosine and their correlation factors in type 2 diabetic patients]. Wei Sheng Yan Jiu. 2009; 38(4):433-6. View

Jafri A, Agarwal R, Iezhitsa I, Agarwal P, Spasov A, Ozerov A . Protective effect of magnesium acetyltaurate and taurine against NMDA-induced retinal damage involves reduced nitrosative stress. Mol Vis. 2018; 24:495-508. PMC: 6066271. View

10.

Murdaugh L, Wang Z, Del Priore L, Dillon J, Gaillard E . Age-related accumulation of 3-nitrotyrosine and nitro-A2E in human Bruch's membrane. Exp Eye Res. 2010; 90(5):564-71. DOI: 10.1016/j.exer.2010.01.014. View

11.

Kauppinen A . Introduction to the multi-author review on macular degeneration. Cell Mol Life Sci. 2020; 77(5):779-780. PMC: 7058669. DOI: 10.1007/s00018-019-03418-5. View

12.

Icel E, Icel A, Ucak T, Karakurt Y, Elpeze B, Cimen F . The effects of lycopene on alloxan induced diabetic optic neuropathy. Cutan Ocul Toxicol. 2018; 38(1):88-92. DOI: 10.1080/15569527.2018.1530258. View

13.

Shen X, Li W . High-mobility group box 1 protein and its role in severe acute pancreatitis. World J Gastroenterol. 2015; 21(5):1424-35. PMC: 4316085. DOI: 10.3748/wjg.v21.i5.1424. View

14.

Wang Z, Paik D, Del Priore L, Burch R, Gaillard E . Nitrite-modified extracellular matrix proteins deleteriously affect retinal pigment epithelial cell function and viability: a comparison study with nonenzymatic glycation mechanisms. Curr Eye Res. 2005; 30(8):691-702. DOI: 10.1080/02713680590968259. View

15.

Ferris 3rd F, Wilkinson C, Bird A, Chakravarthy U, Chew E, Csaky K . Clinical classification of age-related macular degeneration. Ophthalmology. 2013; 120(4):844-51. PMC: 11551519. DOI: 10.1016/j.ophtha.2012.10.036. View

16.

Datta S, Cano M, Ebrahimi K, Wang L, Handa J . The impact of oxidative stress and inflammation on RPE degeneration in non-neovascular AMD. Prog Retin Eye Res. 2017; 60:201-218. PMC: 5600827. DOI: 10.1016/j.preteyeres.2017.03.002. View

17.

Sakamoto K, Mizuta A, Fujimura K, Kurauchi Y, Mori A, Nakahara T . High-mobility group Box-1 is involved in NMDA-induced retinal injury the in rat retina. Exp Eye Res. 2015; 137:63-70. DOI: 10.1016/j.exer.2015.06.003. View

18.

Erel O . A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004; 37(4):277-85. DOI: 10.1016/j.clinbiochem.2003.11.015. View

19.

Bandookwala M, Sengupta P . 3-Nitrotyrosine: a versatile oxidative stress biomarker for major neurodegenerative diseases. Int J Neurosci. 2020; 130(10):1047-1062. DOI: 10.1080/00207454.2020.1713776. View

20.

Murakami Y, Ikeda Y, Nakatake S, Tachibana T, Fujiwara K, Yoshida N . Necrotic enlargement of cone photoreceptor cells and the release of high-mobility group box-1 in retinitis pigmentosa. Cell Death Discov. 2016; 1:15058. PMC: 4979449. DOI: 10.1038/cddiscovery.2015.58. View