Carnosine Supplementation and Retinal Oxidative Parameters in a High-calorie Diet Rat Model

Overview

Journal BMC Ophthalmol

Publisher Biomed Central

Specialty Ophthalmology

Date 2023 Dec 9

PMID 38066465

Authors

Rogil Jose de Almeida Torres

Fernando Moreto

Andrea Luchini

Rogerio Joao de Almeida Torres

Sofia Pimentel Longo

Ricardo Aurino Pinho

Seigo Nagashima

Lucia de Noronha

Artur Junio Togneri Ferron

Carol Cristina Vagula de Almeida Silva

Camila Renata Correa

Giancarlo Aldini

Ana Lucia Anjos Ferreira

Affiliations

Soon will be listed here.

Abstract

Background: To assess oxidative effects induced by a high-calorie diet on the retina of Wistar rats and test the antioxidative effects of carnosine supplementation.

Methods: Wistar rats were randomly divided into the following groups: standard diet (SD), high-calorie diet (HcD), standard diet + carnosine (SD + Car), and high-calorie diet + carnosine (HcD + Car). The body weight, adiposity index, plasma glucose, total lipids, high-density lipoprotein (HDL), low-density lipoprotein (LDL), uric acid, creatinine, and triglycerides of the animals were evaluated. The retinas were analyzed for markers of oxidative stress. Hydrogen peroxide production was assessed by 2',7'-dichlorodihydrofluorescein diacetate (DCF) oxidation. The total glutathione (tGSH), total antioxidant capacity (TAC), protein carbonyl, and sulfhydryl groups of the antioxidant system were analyzed.

Results: TAC levels increased in the retinas of the SD + Car group compared to the SD group (p < 0.05) and in the HcD + Car group compared to the HcD group (p < 0.05). The levels of GSH and the GSSH:GSSG ratio were increased in the HcD + Car group compared to the SD + Car group (p < 0.05). An increase in the retinal carbonyl content was observed in the HcD group compared to the SD group (p < 0.05) and in the HcD + Car group compared to the SD + Car group (p < 0.05). A high-calorie diet (HcD) was also associated with a decrease in retinal sulfhydryl-type levels compared to the SD group (p < 0.05).

Conclusion: The results suggest that feeding a high-calorie diet to rats can promote an increase in carbonyl content and a reduction in sulfhydryl groups in their retinas. The administration of carnosine was not effective in attenuating these oxidative markers.

Trial Registration: Animal Ethics Committee of Botucatu Medical School - Certificate number 1292/2019.

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