Identification and Characterization of an Oligopeptidase Gene Negatively Related to Biofilm Formation

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2016 Oct 8

PMID 27713736

Citations 13

Authors

Hechao Du

Maoda Pang

Yuhao Dong

Yafeng Wu

Nannan Wang

Jin Liu

Furqan Awan

Chengping Lu

Yongjie Liu

Affiliations

Soon will be listed here.

Abstract

Bacterial biofilms are involved in adaptation to complex environments and are responsible for persistent bacterial infections. Biofilm formation is a highly complex process during which multifarious genes work together regularly. In this study, we screened the EZ-Tn5 transposon mutant library to identify genes involved in biofilm formation of . A total of 24 biofilm-associated genes were identified, the majority of which encoded proteins related to cell structure, transcription and translation, gene regulation, growth and metabolism. The mutant strain TM90, in which a gene encoding oligopeptidase F () was disturbed, showed significant upregulation of biofilm formation compared to the parental strain. The TM90 colony phenotype was smaller, more transparent, and splendent. The adhesive ability of TM90 to HEp-2 cells was significantly increased compared with the parental strain. Fifty percent lethal dose (LD) determinations in zebrafish demonstrated that the enhanced-biofilm mutant TM90 was highly attenuated relative to the wild-type strain. In conclusion, the gene is demonstrated for the first time to be a negative factor for biofilm formation and is involved in pathogenicity.

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