Molecular and Virulence Characterization of Highly Prevalent Streptococcus Agalactiae Circulated in Bovine Dairy Herds

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2017 Oct 18

PMID 29037262

Citations 22

Authors

Maoda Pang

Lichang Sun

Tao He

Hongdu Bao

Lili Zhang

Yan Zhou

Hui Zhang

Ruicheng Wei

Yongjie Liu

Ran Wang

Affiliations

Soon will be listed here.

Abstract

Bovine mastitis caused by Streptococcus agalactiae continues to be one of the major veterinary and economic issues in certain areas of the world. The more prevalent S. agalactiae strains that cause bovine mastitis in China dairy farms belong to a number of bovine-adapted sequence types (STs) ST67, ST103 and ST568. However, it is unknown why these STs can emerge as highly prevalent clones in bovine dairy farms. Here, to determine if a variety of virulence characteristics were associated with these highly prevalent STs, the molecular and virulence characterization of 116 strains isolated from bovine, human, fish and environment were analyzed. Our data showed that all bovine-adapted strains could be assigned to capsular genotype Ia or II, and carried pilus island 2b, and lactose operon. Importantly, we demonstrated that the growth ability in milk, biofilm formation ability and adhesion ability to bovine mammary epithelial cells (BMECs) were significantly higher for all bovine-adapted strains compared to strains from other origins. Additionally, ST103 and ST568 strains exhibited significantly higher hemolytic activity and cytotoxicity than ST67 strains. In conclusion, our study provides substantial evidence for the hypothesis that the virulence characteristics including efficient growth in milk, elevated biofilm formation ability, together with strong adhesion ability might have favored the high prevalence of the STs in the bovine environment, whereas the hemolytic activity and cytotoxicity were not the crucial characteristics.

Citing Articles

Non- Staphylococci Cause the Spontaneous Cure or Persistent Infection of Major Bovine Mastitis Pathogens in the Murine Mammary Glands.

Suriyasathaporn W, Kongkaew A, Intanon M, Srithanasuwan A, Saipinta D, Pangprasit N Animals (Basel). 2024; 14(23).

PMID: 39682491 PMC: 11639905. DOI: 10.3390/ani14233526.

Characterization of Virulence Factors and Antimicrobial Susceptibility of Associated with Bovine Mastitis Cases in Thailand.

Wataradee S, Boonserm T, Samngamnim S, Ajariyakhajorn K Animals (Basel). 2024; 14(3).

PMID: 38338090 PMC: 10854646. DOI: 10.3390/ani14030447.

Genotypic and antimicrobial susceptibility of causing bovine mastitis in the central region of Thailand.

Wataradee S, Samngamnim S, Boonserm T, Ajariyakhajorn K Front Vet Sci. 2023; 10:1250436.

PMID: 38026633 PMC: 10666187. DOI: 10.3389/fvets.2023.1250436.

Antimicrobial Resistance and Virulence Genes of Isolated from Mastitis Milk Samples in China.

Zhao Y, Shao W, Wang F, Ma J, Chen H, Wang S J Vet Res. 2023; 66(4):581-590.

PMID: 36846045 PMC: 9944998. DOI: 10.2478/jvetres-2022-0069.

Relationship between Biofilm Production and High Somatic Cell Count in Isolated from Milk of Cows with Subclinical Mastitis.

Bonsaglia E, Rossi R, Latosinski G, Rossi B, Campos F, Junior A Pathogens. 2023; 12(2).

PMID: 36839583 PMC: 9966988. DOI: 10.3390/pathogens12020311.

References

Pang M, Jiang J, Xie X, Wu Y, Dong Y, Kwok A . Novel insights into the pathogenicity of epidemic Aeromonas hydrophila ST251 clones from comparative genomics. Sci Rep. 2015; 5:9833. PMC: 4444815. DOI: 10.1038/srep09833. View

Imperi M, Pataracchia M, Alfarone G, Baldassarri L, Orefici G, Creti R . A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae. J Microbiol Methods. 2009; 80(2):212-4. DOI: 10.1016/j.mimet.2009.11.010. View

Rosini R, Margarit I . Biofilm formation by Streptococcus agalactiae: influence of environmental conditions and implicated virulence factors. Front Cell Infect Microbiol. 2015; 5:6. PMC: 4316791. DOI: 10.3389/fcimb.2015.00006. View

Silanikove N, Leitner G, Merin U . The Interrelationships between Lactose Intolerance and the Modern Dairy Industry: Global Perspectives in Evolutional and Historical Backgrounds. Nutrients. 2015; 7(9):7312-31. PMC: 4586535. DOI: 10.3390/nu7095340. View

Kayansamruaj P, Pirarat N, Kondo H, Hirono I, Rodkhum C . Genomic comparison between pathogenic Streptococcus agalactiae isolated from Nile tilapia in Thailand and fish-derived ST7 strains. Infect Genet Evol. 2015; 36:307-314. DOI: 10.1016/j.meegid.2015.10.009. View

Ferretti J, McShan W, Ajdic D, Savic D, Savic G, Lyon K . Complete genome sequence of an M1 strain of Streptococcus pyogenes. Proc Natl Acad Sci U S A. 2001; 98(8):4658-63. PMC: 31890. DOI: 10.1073/pnas.071559398. View

Herigstad B, Hamilton M, Heersink J . How to optimize the drop plate method for enumerating bacteria. J Microbiol Methods. 2001; 44(2):121-9. DOI: 10.1016/s0167-7012(00)00241-4. View

Costerton J, Stewart P, Greenberg E . Bacterial biofilms: a common cause of persistent infections. Science. 1999; 284(5418):1318-22. DOI: 10.1126/science.284.5418.1318. View

Lyhs U, Kulkas L, Katholm J, Persson Waller K, Saha K, Tomusk R . Streptococcus agalactiae Serotype IV in Humans and Cattle, Northern Europe. Emerg Infect Dis. 2016; 22(12):2097-2103. PMC: 5189126. DOI: 10.3201/eid2212.151447. View

10.

Konto-Ghiorghi Y, Mairey E, Mallet A, Dumenil G, Caliot E, Trieu-Cuot P . Dual role for pilus in adherence to epithelial cells and biofilm formation in Streptococcus agalactiae. PLoS Pathog. 2009; 5(5):e1000422. PMC: 2674936. DOI: 10.1371/journal.ppat.1000422. View

11.

Lauer P, Rinaudo C, Soriani M, Margarit I, Maione D, Rosini R . Genome analysis reveals pili in Group B Streptococcus. Science. 2005; 309(5731):105. DOI: 10.1126/science.1111563. View

12.

Budd K, Mitchell J, Keane O . Lineage associated expression of virulence traits in bovine-adapted Staphylococcus aureus. Vet Microbiol. 2016; 189:24-31. DOI: 10.1016/j.vetmic.2016.04.013. View

13.

Leclercq S, Sullivan M, Ipe D, Smith J, Cripps A, Ulett G . Pathogenesis of Streptococcus urinary tract infection depends on bacterial strain and β-hemolysin/cytolysin that mediates cytotoxicity, cytokine synthesis, inflammation and virulence. Sci Rep. 2016; 6:29000. PMC: 4935997. DOI: 10.1038/srep29000. View

14.

Papazafiri P, Avlonitis N, Angelou P, Calogeropoulou T, Koufaki M, Scoulica E . Structure-activity relationships of antineoplastic ring-substituted ether phospholipid derivatives. Cancer Chemother Pharmacol. 2005; 56(3):261-70. DOI: 10.1007/s00280-004-0935-6. View

15.

Du H, Pang M, Dong Y, Wu Y, Wang N, Liu J . Identification and Characterization of an Oligopeptidase Gene Negatively Related to Biofilm Formation. Front Microbiol. 2016; 7:1497. PMC: 5032638. DOI: 10.3389/fmicb.2016.01497. View

16.

Jones N, Bohnsack J, Takahashi S, Oliver K, Chan M, Kunst F . Multilocus sequence typing system for group B streptococcus. J Clin Microbiol. 2003; 41(6):2530-6. PMC: 156480. DOI: 10.1128/JCM.41.6.2530-2536.2003. View

17.

Richards V, Choi S, Bitar P, Gurjar A, Stanhope M . Transcriptomic and genomic evidence for Streptococcus agalactiae adaptation to the bovine environment. BMC Genomics. 2013; 14:920. PMC: 3890567. DOI: 10.1186/1471-2164-14-920. View

18.

Fischer A, Liljander A, Kaspar H, Muriuki C, Fuxelius H, Bongcam-Rudloff E . Camel Streptococcus agalactiae populations are associated with specific disease complexes and acquired the tetracycline resistance gene tetM via a Tn916-like element. Vet Res. 2013; 44:86. PMC: 3850529. DOI: 10.1186/1297-9716-44-86. View

19.

Giannouli M, Antunes L, Marchetti V, Triassi M, Visca P, Zarrilli R . Virulence-related traits of epidemic Acinetobacter baumannii strains belonging to the international clonal lineages I-III and to the emerging genotypes ST25 and ST78. BMC Infect Dis. 2013; 13:282. PMC: 3691691. DOI: 10.1186/1471-2334-13-282. View

20.

Weisburg W, Barns S, Pelletier D, Lane D . 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991; 173(2):697-703. PMC: 207061. DOI: 10.1128/jb.173.2.697-703.1991. View