Monitoring the Synthesis of Biomolecules Using Mass Spectrometry

Overview

Journal Philos Trans A Math Phys Eng Sci

Specialties Biomedical Engineering
Biophysics
Public Health

Date 2016 Sep 21

PMID 27644976

Citations 8

Authors

Masaru Miyagi

Takhar Kasumov

Affiliations

Soon will be listed here.

Abstract

The controlled and selective synthesis/clearance of biomolecules is critical for most cellular processes. In most high-throughput 'omics' studies, we measure the static quantities of only one class of biomolecules (e.g. DNA, mRNA, proteins or metabolites). It is, however, important to recognize that biological systems are highly dynamic in which biomolecules are continuously renewed and different classes of biomolecules interact and affect each other's production/clearance. Therefore, it is necessary to measure the turnover of diverse classes of biomolecules to understand the dynamic nature of biological systems. Herein, we explain why the kinetic analysis of a diverse range of biomolecules is important and how such an analysis can be done. We argue that heavy water ((2)H2O) could be a universal tracer for monitoring the synthesis of biomolecules on a global scale.This article is part of the themed issue 'Quantitative mass spectrometry'.

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