Diffusion Pseudotime Robustly Reconstructs Lineage Branching

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2016 Aug 30

PMID 27571553

Citations 576

Authors

Laleh Haghverdi

Maren Buttner

F Alexander Wolf

Florian Buettner

Fabian J Theis

Affiliations

Soon will be listed here.

Abstract

The temporal order of differentiating cells is intrinsically encoded in their single-cell expression profiles. We describe an efficient way to robustly estimate this order according to diffusion pseudotime (DPT), which measures transitions between cells using diffusion-like random walks. Our DPT software implementations make it possible to reconstruct the developmental progression of cells and identify transient or metastable states, branching decisions and differentiation endpoints.

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