Trajectories of Cell-cycle Progression from Fixed Cell Populations

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2015 Aug 25

PMID 26301842

Citations 51

Authors

Gabriele Gut

Michelle D Tadmor

Dana Peer

Lucas Pelkmans

Prisca Liberali

Affiliations

Soon will be listed here.

Abstract

An accurate dissection of sources of cell-to-cell variability is crucial for quantitative biology at the single-cell level but has been challenging for the cell cycle. We present Cycler, a robust method that constructs a continuous trajectory of cell-cycle progression from images of fixed cells. Cycler handles heterogeneous microenvironments and does not require perturbations or genetic markers, making it generally applicable to quantifying multiple sources of cell-to-cell variability in mammalian cells.

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