Electric Pulses: a Flexible Tool to Manipulate Cytosolic Calcium Concentrations and Generate Spontaneous-like Calcium Oscillations in Mesenchymal Stem Cells

Overview

Journal Sci Rep

Specialty Science

Date 2016 Aug 27

PMID 27561994

Citations 10

Authors

Marie-Amelie de Menorval

Franck M Andre

Aude Silve

Claire Dalmay

Olivier Francais

Bruno Le Pioufle

Lluis M Mir

Affiliations

Soon will be listed here.

Abstract

Human adipose mesenchymal stem cells (haMSCs) are multipotent adult stem cells of great interest in regenerative medicine or oncology. They present spontaneous calcium oscillations related to cell cycle progression or differentiation but the correlation between these events is still unclear. Indeed, it is difficult to mimic haMSCs spontaneous calcium oscillations with chemical means. Pulsed electric fields (PEFs) can permeabilise plasma and/or organelles membranes depending on the applied pulses and therefore generate cytosolic calcium peaks by recruiting calcium from the external medium or from internal stores. We show that it is possible to mimic haMSCs spontaneous calcium oscillations (same amplitude, duration and shape) using 100 μs PEFs or 10 ns PEFs. We propose a model that explains the experimental situations reported. PEFs can therefore be a flexible tool to manipulate cytosolic calcium concentrations. This tool, that can be switched on and off instantaneously, contrary to chemicals agents, can be very useful to investigate the role of calcium oscillations in cell physiology and/or to manipulate cell fate.

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