A Wide-band Bio-chip for Real-time Optical Detection of Bioelectromagnetic Interactions with Cells

Overview

Journal Sci Rep

Specialty Science

Date 2018 Mar 24

PMID 29568067

Citations 4

Authors

Caterina Merla

Micaela Liberti

Paolo Marracino

Adeline Muscat

Antoine Azan

Francesca Apollonio

Lluis M Mir

Affiliations

Soon will be listed here.

Abstract

The analytical and numerical design, implementation, and experimental validation of a new grounded closed coplanar waveguide for wide-band electromagnetic exposures of cells and their optical detection in real-time is reported. The realized device fulfills high-quality requirements for novel bioelectromagnetic experiments, involving elevated temporal and spatial resolutions. Excellent performances in terms of matching bandwidth (less than -10 dB up to at least 3 GHz), emission (below 1 × 10 W/m) and efficiency (around 1) have been obtained as revealed by both numerical simulations and experimental measurements. A low spatial electric field inhomogeneity (coefficient of variation of around 10 %) has been achieved within the cell solutions filling the polydimethylsiloxane reservoir of the conceived device. This original bio-chip based on the grounded closed coplanar waveguide concept opens new possibilities for the development of controlled experiments combining electromagnetic exposures and sophisticated imaging using optical spectroscopic techniques.

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References

Batista Napotnik T, Rebersek M, Vernier P, Mali B, Miklavcic D . Effects of high voltage nanosecond electric pulses on eukaryotic cells (in vitro): A systematic review. Bioelectrochemistry. 2016; 110:1-12. DOI: 10.1016/j.bioelechem.2016.02.011. View

Sadik M, Li J, Shan J, Shreiber D, Lin H . Quantification of propidium iodide delivery using millisecond electric pulses: experiments. Biochim Biophys Acta. 2013; 1828(4):1322-8. DOI: 10.1016/j.bbamem.2013.01.002. View

Carr L, Bardet S, Burke R, Arnaud-Cormos D, Leveque P, OConnor R . Calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields in U87 human glioblastoma cells. Sci Rep. 2017; 7:41267. PMC: 5259788. DOI: 10.1038/srep41267. View

Azan A, Untereiner V, Gobinet C, Sockalingum G, Breton M, Piot O . Demonstration of the Protein Involvement in Cell Electropermeabilization using Confocal Raman Microspectroscopy. Sci Rep. 2017; 7:40448. PMC: 5244372. DOI: 10.1038/srep40448. View

Lesueur L, Mir L, Andre F . Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro. Mol Ther Nucleic Acids. 2016; 5:e291. PMC: 5014460. DOI: 10.1038/mtna.2016.4. View

Breton M, Delemotte L, Silve A, Mir L, Tarek M . Transport of siRNA through lipid membranes driven by nanosecond electric pulses: an experimental and computational study. J Am Chem Soc. 2012; 134(34):13938-41. DOI: 10.1021/ja3052365. View

Nuccitelli R, Huynh J, Lui K, Wood R, Kreis M, Athos B . Nanoelectroablation of human pancreatic carcinoma in a murine xenograft model without recurrence. Int J Cancer. 2012; 132(8):1933-9. PMC: 3554856. DOI: 10.1002/ijc.27860. View

Calvet C, Mir L . The promising alliance of anti-cancer electrochemotherapy with immunotherapy. Cancer Metastasis Rev. 2016; 35(2):165-77. PMC: 4911376. DOI: 10.1007/s10555-016-9615-3. View

Pucihar G, Kotnik T, Miklavcic D . Measuring the induced membrane voltage with Di-8-ANEPPS. J Vis Exp. 2009; (33). PMC: 3142892. DOI: 10.3791/1659. View

10.

Breton M, Mir L . Investigation of the chemical mechanisms involved in the electropulsation of membranes at the molecular level. Bioelectrochemistry. 2017; 119:76-83. DOI: 10.1016/j.bioelechem.2017.09.005. View

11.

Craviso G, Choe S, Chatterjee I, Vernier P . Modulation of intracellular Ca2+ levels in chromaffin cells by nanoelectropulses. Bioelectrochemistry. 2011; 87:244-52. DOI: 10.1016/j.bioelechem.2011.11.016. View

12.

Vernier P, Sun Y, Marcu L, Salemi S, Craft C, Gundersen M . Calcium bursts induced by nanosecond electric pulses. Biochem Biophys Res Commun. 2003; 310(2):286-95. DOI: 10.1016/j.bbrc.2003.08.140. View

13.

Denzi A, Valle E, Apollonio F, Breton M, Mir L, Liberti M . Exploring the Applicability of Nano-Poration for Remote Control in Smart Drug Delivery Systems. J Membr Biol. 2016; 250(1):31-40. DOI: 10.1007/s00232-016-9922-1. View

14.

Merla C, Apollonio F, Paffi A, Marino C, Vernier P, Liberti M . Monopole patch antenna for in vivo exposure to nanosecond pulsed electric fields. Med Biol Eng Comput. 2016; 55(7):1073-1083. DOI: 10.1007/s11517-016-1547-0. View

15.

Bardet S, Carr L, Soueid M, Arnaud-Cormos D, Leveque P, OConnor R . Multiphoton imaging reveals that nanosecond pulsed electric fields collapse tumor and normal vascular perfusion in human glioblastoma xenografts. Sci Rep. 2016; 6:34443. PMC: 5048165. DOI: 10.1038/srep34443. View

16.

Barnes R, Roth C, Beier H, Noojin G, Valdez C, Bixler J . Probe beam deflection optical imaging of thermal and mechanical phenomena resulting from nanosecond electric pulse (nsEP) exposure in-vitro. Opt Express. 2017; 25(6):6621-6643. DOI: 10.1364/OE.25.006621. View

17.

Thompson G, Roth C, Kuipers M, Tolstykh G, Beier H, Ibey B . Permeabilization of the nuclear envelope following nanosecond pulsed electric field exposure. Biochem Biophys Res Commun. 2016; 470(1):35-40. DOI: 10.1016/j.bbrc.2015.12.092. View

18.

Sage C, Burgio E . Electromagnetic Fields, Pulsed Radiofrequency Radiation, and Epigenetics: How Wireless Technologies May Affect Childhood Development. Child Dev. 2017; 89(1):129-136. DOI: 10.1111/cdev.12824. View

19.

Rangel M, Chaible L, Nagamine M, Mennecier G, Cogliati B, de Oliveira K . Electroporation transiently decreases GJB2 (connexin 26) expression in B16/BL6 melanoma cell line. J Membr Biol. 2014; 248(1):47-52. DOI: 10.1007/s00232-014-9735-z. View

20.

Casciola M, Bonhenry D, Liberti M, Apollonio F, Tarek M . A molecular dynamic study of cholesterol rich lipid membranes: comparison of electroporation protocols. Bioelectrochemistry. 2014; 100:11-7. DOI: 10.1016/j.bioelechem.2014.03.009. View