Technological and Theoretical Aspects for Testing Electroporation on Liposomes

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2017 Apr 11

PMID 28393078

Citations 5

Authors

Agnese Denzi

Elena Della Valle

Gianluca Esposito

Lluis M Mir

Francesca Apollonio

Micaela Liberti

Affiliations

Soon will be listed here.

Abstract

Recently, the use of nanometer liposomes as nanocarriers in drug delivery systems mediated by nanoelectroporation has been proposed. This technique takes advantage of the possibility of simultaneously electroporating liposomes and cell membrane with 10-nanosecond pulsed electric fields facilitating the release of the drug from the liposomes and at the same time its uptake by the cells. In this paper the design and characterization of a 10 exposure system is presented, for liposomes electroporation purposes. The design and the characterization of the applicator have been carried out choosing an electroporation cuvette with 1 mm gap between the electrodes. The structure efficiency has been evaluated at different experimental conditions by changing the solution conductivity from 0.25 to 1.6 S/m. With the aim to analyze the influence of device performances on the liposomes electroporation, microdosimetric simulations have been performed considering liposomes of 200 and 400 nm of dimension with different inner and outer conductivity (from 0.05 to 1.6 S/m) in order to identify the voltage needed for their poration.

Citing Articles

Electroporation in Head-and-Neck Cancer: An Innovative Approach with Immunotherapy and Nanotechnology Combination.

Pisani S, Bertino G, Prina-Mello A, Locati L, Mauramati S, Genta I Cancers (Basel). 2022; 14(21).

PMID: 36358782 PMC: 9658293. DOI: 10.3390/cancers14215363.

Pulsed Electric Fields Promote Liposome Buddings.

Urabe G, Shimada M, Ogata T, Katsuki S Bioelectricity. 2021; 3(1):68-76.

PMID: 34476378 PMC: 8390777. DOI: 10.1089/bioe.2020.0016.

Confocal Microscopy Improves 3D Microdosimetry Applied to Nanoporation Experiments Targeting Endoplasmic Reticulum.

De Angelis A, Denzi A, Merla C, Andre F, Mir L, Apollonio F Front Bioeng Biotechnol. 2020; 8:552261.

PMID: 33072718 PMC: 7537786. DOI: 10.3389/fbioe.2020.552261.

Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments.

Caramazza L, Nardoni M, De Angelis A, Paolicelli P, Liberti M, Apollonio F Front Bioeng Biotechnol. 2020; 8:819.

PMID: 32793572 PMC: 7390969. DOI: 10.3389/fbioe.2020.00819.

Possible molecular and cellular mechanisms at the basis of atmospheric electromagnetic field bioeffects.

Cifra M, Apollonio F, Liberti M, Garcia-Sanchez T, Mir L Int J Biometeorol. 2020; 65(1):59-67.

PMID: 32335726 PMC: 7782448. DOI: 10.1007/s00484-020-01885-1.

References

Batista Napotnik T, Rebersek M, Vernier P, Mali B, Miklavcic D . Effects of high voltage nanosecond electric pulses on eukaryotic cells (in vitro): A systematic review. Bioelectrochemistry. 2016; 110:1-12. DOI: 10.1016/j.bioelechem.2016.02.011. View

Denzi A, Strigari L, Di Filippo F, Botti C, Di Filippo S, Perracchio L . Modeling the positioning of single needle electrodes for the treatment of breast cancer in a clinical case. Biomed Eng Online. 2015; 14 Suppl 3:S1. PMC: 4565101. DOI: 10.1186/1475-925X-14-S3-S1. View

Cadossi R, Ronchetti M, Cadossi M . Locally enhanced chemotherapy by electroporation: clinical experiences and perspective of use of electrochemotherapy. Future Oncol. 2014; 10(5):877-90. DOI: 10.2217/fon.13.235. View

Mir L, Bureau M, Gehl J, Rangara R, Rouy D, CAILLAUD J . High-efficiency gene transfer into skeletal muscle mediated by electric pulses. Proc Natl Acad Sci U S A. 1999; 96(8):4262-7. PMC: 16320. DOI: 10.1073/pnas.96.8.4262. View

Casciola M, Tarek M . A molecular insight into the electro-transfer of small molecules through electropores driven by electric fields. Biochim Biophys Acta. 2016; 1858(10):2278-2289. DOI: 10.1016/j.bbamem.2016.03.022. View

Breton M, Delemotte L, Silve A, Mir L, Tarek M . Transport of siRNA through lipid membranes driven by nanosecond electric pulses: an experimental and computational study. J Am Chem Soc. 2012; 134(34):13938-41. DOI: 10.1021/ja3052365. View

Denzi A, Valle E, Apollonio F, Breton M, Mir L, Liberti M . Exploring the Applicability of Nano-Poration for Remote Control in Smart Drug Delivery Systems. J Membr Biol. 2016; 250(1):31-40. DOI: 10.1007/s00232-016-9922-1. View

Kitano H . Computational systems biology. Nature. 2002; 420(6912):206-10. DOI: 10.1038/nature01254. View

Marracino P, Castellani F, Vernier P, Liberti M, Apollonio F . Geometrical Characterization of an Electropore from Water Positional Fluctuations. J Membr Biol. 2016; 250(1):11-19. DOI: 10.1007/s00232-016-9917-y. View

10.

Kotnik T, Pucihar G, Miklavcic D . Induced transmembrane voltage and its correlation with electroporation-mediated molecular transport. J Membr Biol. 2010; 236(1):3-13. DOI: 10.1007/s00232-010-9279-9. View

11.

Kitano H . Systems biology: a brief overview. Science. 2002; 295(5560):1662-4. DOI: 10.1126/science.1069492. View

12.

Joshi R, Schoenbach K . Bioelectric effects of intense ultrashort pulses. Crit Rev Biomed Eng. 2010; 38(3):255-304. DOI: 10.1615/critrevbiomedeng.v38.i3.20. View

13.

de Menorval M, Andre F, Silve A, Dalmay C, Francais O, Le Pioufle B . Electric pulses: a flexible tool to manipulate cytosolic calcium concentrations and generate spontaneous-like calcium oscillations in mesenchymal stem cells. Sci Rep. 2016; 6:32331. PMC: 4999807. DOI: 10.1038/srep32331. View

14.

Kenaan M, El Amari S, Silve A, Merla C, Mir L, Couderc V . Characterization of a 50- Ω exposure setup for high-voltage nanosecond pulsed electric field bioexperiments. IEEE Trans Biomed Eng. 2010; 58(1):207-14. DOI: 10.1109/TBME.2010.2081670. View

15.

Arnaud-Cormos D, Leveque P, Wu Y, Sanders J, Gundersen M, Vernier P . Microchamber setup characterization for nanosecond pulsed electric field exposure. IEEE Trans Biomed Eng. 2011; 58(6):1656-62. DOI: 10.1109/TBME.2011.2108298. View

16.

Silve A, Leray I, Poignard C, Mir L . Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses. Sci Rep. 2016; 6:19957. PMC: 4734290. DOI: 10.1038/srep19957. View

17.

Ibey B, Roth C, Pakhomov A, Bernhard J, Wilmink G, Pakhomova O . Dose-dependent thresholds of 10-ns electric pulse induced plasma membrane disruption and cytotoxicity in multiple cell lines. PLoS One. 2011; 6(1):e15642. PMC: 3027629. DOI: 10.1371/journal.pone.0015642. View

18.

Dalmay C, Villemejane J, Joubert V, Silve A, Arnaud-Cormos D, Francais O . A microfluidic biochip for the nanoporation of living cells. Biosens Bioelectron. 2011; 26(12):4649-55. DOI: 10.1016/j.bios.2011.03.020. View

19.

Silve A, Leray I, Mir L . Demonstration of cell membrane permeabilization to medium-sized molecules caused by a single 10 ns electric pulse. Bioelectrochemistry. 2011; 87:260-4. DOI: 10.1016/j.bioelechem.2011.10.002. View

20.

Langus J, Kranjc M, Kos B, Sustar T, Miklavcic D . Dynamic finite-element model for efficient modelling of electric currents in electroporated tissue. Sci Rep. 2016; 6:26409. PMC: 4876422. DOI: 10.1038/srep26409. View