Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2016 Aug 25

PMID 27554600

Citations 68

Authors

Piyush K Jain

Vyas Ramanan

Arnout G Schepers

Nisha S Dalvie

Apekshya Panda

Heather E Fleming

Sangeeta N Bhatia

Affiliations

Soon will be listed here.

Abstract

The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations.

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References

Tang X, Dmochowski I . Regulating gene expression with light-activated oligonucleotides. Mol Biosyst. 2007; 3(2):100-10. DOI: 10.1039/b614349k. View

Dudani J, Jain P, Kwong G, Stevens K, Bhatia S . Photoactivated Spatiotemporally-Responsive Nanosensors of in Vivo Protease Activity. ACS Nano. 2015; 9(12):11708-17. PMC: 5588683. DOI: 10.1021/acsnano.5b05946. View

Walter R . The role of CD33 as therapeutic target in acute myeloid leukemia. Expert Opin Ther Targets. 2014; 18(7):715-8. DOI: 10.1517/14728222.2014.909413. View

Hemphill J, Borchardt E, Brown K, Asokan A, Deiters A . Optical Control of CRISPR/Cas9 Gene Editing. J Am Chem Soc. 2015; 137(17):5642-5. PMC: 4919123. DOI: 10.1021/ja512664v. View

Zetsche B, Volz S, Zhang F . A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015; 33(2):139-42. PMC: 4503468. DOI: 10.1038/nbt.3149. View

Liu Q, Wang M, Hu Y, Xing H, Chen X, Zhang Y . Significance of CD71 expression by flow cytometry in diagnosis of acute leukemia. Leuk Lymphoma. 2013; 55(4):892-8. DOI: 10.3109/10428194.2013.819100. View

Ran F, Hsu P, Wright J, Agarwala V, Scott D, Zhang F . Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013; 8(11):2281-2308. PMC: 3969860. DOI: 10.1038/nprot.2013.143. View

Doudna J, Charpentier E . Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science. 2014; 346(6213):1258096. DOI: 10.1126/science.1258096. View

Ran F, Cong L, Yan W, Scott D, Gootenberg J, Kriz A . In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015; 520(7546):186-91. PMC: 4393360. DOI: 10.1038/nature14299. View

10.

Shestopalov I, Sinha S, Chen J . Light-controlled gene silencing in zebrafish embryos. Nat Chem Biol. 2007; 3(10):650-1. DOI: 10.1038/nchembio.2007.30. View

11.

Li L, Tong R, Chu H, Wang W, Langer R, Kohane D . Aptamer photoregulation in vivo. Proc Natl Acad Sci U S A. 2014; 111(48):17099-103. PMC: 4260580. DOI: 10.1073/pnas.1420105111. View

12.

Hemphill J, Liu Q, Uprety R, Samanta S, Tsang M, Juliano R . Conditional control of alternative splicing through light-triggered splice-switching oligonucleotides. J Am Chem Soc. 2015; 137(10):3656-62. PMC: 5545098. DOI: 10.1021/jacs.5b00580. View

13.

Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M . CRISPR-Cas9-based photoactivatable transcription system. Chem Biol. 2015; 22(2):169-74. DOI: 10.1016/j.chembiol.2014.12.011. View

14.

Kleinstiver B, Prew M, Tsai S, Topkar V, Nguyen N, Zheng Z . Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature. 2015; 523(7561):481-5. PMC: 4540238. DOI: 10.1038/nature14592. View

15.

OConnell M, Oakes B, Sternberg S, East-Seletsky A, Kaplan M, Doudna J . Programmable RNA recognition and cleavage by CRISPR/Cas9. Nature. 2014; 516(7530):263-6. PMC: 4268322. DOI: 10.1038/nature13769. View

16.

Ruiz-Arguelles G, San Miguel J . Cell surface markers in multiple myeloma. Mayo Clin Proc. 1994; 69(7):684-90. DOI: 10.1016/s0025-6196(12)61350-0. View

17.

Toyooka T, Ishihama M, Ibuki Y . Phosphorylation of histone H2AX is a powerful tool for detecting chemical photogenotoxicity. J Invest Dermatol. 2011; 131(6):1313-21. DOI: 10.1038/jid.2011.28. View

18.

Jiang F, Zhou K, Ma L, Gressel S, Doudna J . STRUCTURAL BIOLOGY. A Cas9-guide RNA complex preorganized for target DNA recognition. Science. 2015; 348(6242):1477-81. DOI: 10.1126/science.aab1452. View

19.

Zhang F . CRISPR-Cas9: Prospects and Challenges. Hum Gene Ther. 2015; 26(7):409-10. PMC: 4939442. DOI: 10.1089/hum.2015.29002.fzh. View

20.

Sander J, Joung J . CRISPR-Cas systems for editing, regulating and targeting genomes. Nat Biotechnol. 2014; 32(4):347-55. PMC: 4022601. DOI: 10.1038/nbt.2842. View