Norbornene-tetrazine Ligation Chemistry for Controlling RNA-guided CRISPR Systems

Overview

Journal Chem Sci

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 Nov 16

PMID 36382274

Authors

Qianqian Qi

Yutong Zhang

Wei Xiong

Xingyu Liu

Shuangyu Cui

Xiaofang Ye

Kaisong Zhang

Tian Tian

Xiang Zhou

Affiliations

Soon will be listed here.

Abstract

In the present study, norbornene-tetrazine ligation chemistry is harnessed for developing clickable RNA switches in biological contexts. This RNA control strategy is explored with a variety of applications. We further demonstrate the application of RNA-based norbornene-tetrazine ligation chemistry for controlling CRISPR systems. Moreover, the manipulation of gene editing in human cells is accomplished.

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