Plasticity-induced Actin Polymerization in the Dendritic Shaft Regulates Intracellular AMPA Receptor Trafficking

Overview

Journal Elife

Specialty Biology

Date 2024 Aug 15

PMID 39146380

Authors

Victor C Wong

Patrick R Houlihan

Hui Liu

Deepika Walpita

Michael C DeSantis

Zhe Liu

Erin K OShea

Affiliations

Soon will be listed here.

Abstract

AMPA-type receptors (AMPARs) are rapidly inserted into synapses undergoing plasticity to increase synaptic transmission, but it is not fully understood if and how AMPAR-containing vesicles are selectively trafficked to these synapses. Here, we developed a strategy to label AMPAR GluA1 subunits expressed from their endogenous loci in cultured rat hippocampal neurons and characterized the motion of GluA1-containing vesicles using single-particle tracking and mathematical modeling. We find that GluA1-containing vesicles are confined and concentrated near sites of stimulation-induced structural plasticity. We show that confinement is mediated by actin polymerization, which hinders the active transport of GluA1-containing vesicles along the length of the dendritic shaft by modulating the rheological properties of the cytoplasm. Actin polymerization also facilitates myosin-mediated transport of GluA1-containing vesicles to exocytic sites. We conclude that neurons utilize F-actin to increase vesicular GluA1 reservoirs and promote exocytosis proximal to the sites of synaptic activity.

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