MicroRNA-16 is Putatively Involved in the NF-κB Pathway Regulation in Ulcerative Colitis Through Adenosine A2a Receptor (A2aAR) MRNA Targeting

Overview

Journal Sci Rep

Specialty Science

Date 2016 Aug 2

PMID 27476546

Citations 43

Authors

Ting Tian

Yu Zhou

Xiao Feng

Shicai Ye

Hao Wang

Weiyun Wu

Wenkai Tan

Caiyuan Yu

Juxiang Hu

Rong Zheng

Zonghao Chen

Xinyu Pei

Hesheng Luo

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) act as important post-transcriptional regulators of gene expression by targeting the 3'-untranslated region of their target genes. Altered expression of miR-16 is reported in human ulcerative colitis (UC), but its role in the development of the disease remains unclear. Adenosine through adenosine A2a receptor (A2aAR) could inhibit nuclear factor-kappaB (NF-κB) signaling pathway in inflammation. Here we identified overexpression of miR-16 and down-regulation of A2aAR in the colonic mucosa of active UC patients. We demonstrated that miR-16 negatively regulated the expression of the A2aAR at the post-transcriptional level. Furthermore, transfection of miR-16 mimics promoted nuclear translocation of NF-κB p65 protein and expression of pro-inflammatory cytokines, IFN-γ and IL-8 in colonic epithelial cells. Treatment with miR-16 inhibitor could reverse these effects in cells. The A2aAR-mediated effects of miR-16 on the activation of the NF-κB signaling pathway were confirmed by the A2aAR knockdown assay. Our results suggest that miR-16 regulated the immune and inflammatory responses, at least in part, by suppressing the expression of the A2aAR to control the activation of the NF-κB signaling pathway.

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