Conidial Germination in Scedosporium Apiospermum, S. Aurantiacum, S. Minutisporum and Lomentospora Prolificans: Influence of Growth Conditions and Antifungal Susceptibility Profiles

Overview

Journal Mem Inst Oswaldo Cruz

Specialties Parasitology
Tropical Medicine

Date 2016 Jun 30

PMID 27355215

Citations 11

Authors

Thais Pereira de Mello

Ana Carolina Aor

Simone Santiago Carvalho de Oliveira

Marta Helena Branquinha

Andre Luis Souza Dos Santos

Affiliations

Soon will be listed here.

Abstract

In the present study, we have investigated some growth conditions capable of inducing the conidial germination in Scedosporium apiospermum, S. aurantiacum, S. minutisporum and Lomentospora prolificans. Germination in Sabouraud medium (pH 7.0, 37ºC, 5% CO2) showed to be a typically time-dependent event, reaching ~75% in S. minutisporum and > 90% in S. apiospermum, S. aurantiacum and L. prolificans after 4 h. Similar germination rate was observed when conidia were incubated under different media and pHs. Contrarily, temperature and CO2 tension modulated the germination. The isotropic conidial growth (swelling) and germ tube-like projection were evidenced by microscopy and cytometry. Morphometric parameters augmented in a time-dependent fashion, evidencing changes in size and granularity of fungal cells compared with dormant 0 h conidia. In parallel, a clear increase in the mitochondrial activity was measured during the transformation of conidia-into-germinated conidia. Susceptibility profiles to itraconazole, fluconazole, voriconazole, amphotericin B and caspofungin varied regarding each morphotype and each fungal species. Overall, the minimal inhibitory concentrations for hyphae were higher than conidia and germinated conidia, except for caspofungin. Collectively, our study add new data about the conidia-into-hyphae transformation in Scedosporium and Lomentospora species, which is a relevant biological process of these molds directly connected to their antifungal resistance and pathogenicity mechanisms.

Citing Articles

A case of pulmonary infection in a patient with acute myeloid leukemia.

Arjomand A, Myers A, Akella P Respir Med Case Rep. 2024; 51:102071.

PMID: 38974754 PMC: 11225683. DOI: 10.1016/j.rmcr.2024.102071.

Scedosporiosis and lomentosporiosis: modern perspectives on these difficult-to-treat rare mold infections.

Neoh C, Chen S, Lanternier F, Tio S, Halliday C, Kidd S Clin Microbiol Rev. 2024; 37(2):e0000423.

PMID: 38551323 PMC: 11237582. DOI: 10.1128/cmr.00004-23.

The genomes of Scedosporium between environmental challenges and opportunism.

Venice F, Spina F, Davolos D, Ghignone S, Varese G IMA Fungus. 2023; 14(1):25.

PMID: 38049914 PMC: 10694956. DOI: 10.1186/s43008-023-00128-3.

Development and efficacy of tryptophol-containing emulgel for reducing subcutaneous fungal nodules from eumycetoma.

Kitisin T, Muangkaew W, Ampawong S, Sansurin N, Thitipramote N, Sukphopetch P Res Pharm Sci. 2023; 17(6):707-722.

PMID: 36704435 PMC: 9872179. DOI: 10.4103/1735-5362.359437.

Disseminated Infections: A Systematic Review of Reported Cases.

Konsoula A, Agouridis A, Markaki L, Tsioutis C, Spernovasilis N Pathogens. 2023; 12(1).

PMID: 36678415 PMC: 9861501. DOI: 10.3390/pathogens12010067.

References

Dagenais T, Keller N . Pathogenesis of Aspergillus fumigatus in Invasive Aspergillosis. Clin Microbiol Rev. 2009; 22(3):447-65. PMC: 2708386. DOI: 10.1128/CMR.00055-08. View

Lopes L, Rollin-Pinheiro R, Guimaraes A, Bittencourt V, Martinez L, Koba W . Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus. PLoS Negl Trop Dis. 2010; 4(10):e853. PMC: 2957425. DOI: 10.1371/journal.pntd.0000853. View

Silva B, Pinto M, Soares R, Barreto-Bergter E, Santos A . Pseudallescheria boydii releases metallopeptidases capable of cleaving several proteinaceous compounds. Res Microbiol. 2006; 157(5):425-32. DOI: 10.1016/j.resmic.2005.11.010. View

Kantarcioglu A, de Hoog G, Guarro J . Clinical characteristics and epidemiology of pulmonary pseudallescheriasis. Rev Iberoam Micol. 2011; 29(1):1-13. DOI: 10.1016/j.riam.2011.04.002. View

Aimanianda V, Bayry J, Bozza S, Kniemeyer O, Perruccio K, Elluru S . Surface hydrophobin prevents immune recognition of airborne fungal spores. Nature. 2009; 460(7259):1117-21. DOI: 10.1038/nature08264. View

OBryan T . Pseudallescheriasis in the 21st century. Expert Rev Anti Infect Ther. 2005; 3(5):765-73. DOI: 10.1586/14787210.3.5.765. View

Hohl T, Van Epps H, Rivera A, Morgan L, Chen P, Feldmesser M . Aspergillus fumigatus triggers inflammatory responses by stage-specific beta-glucan display. PLoS Pathog. 2005; 1(3):e30. PMC: 1287910. DOI: 10.1371/journal.ppat.0010030. View

Liu Q, Xiao C . Influence of Nutrient and Environmental Factors on Conidial Germination of Potebniamyces pyri. Phytopathology. 2008; 95(5):572-80. DOI: 10.1094/PHYTO-95-0572. View

Lang-Yona N, Levin Y, Dannemiller K, Yarden O, Peccia J, Rudich Y . Changes in atmospheric CO2 influence the allergenicity of Aspergillus fumigatus. Glob Chang Biol. 2013; 19(8):2381-8. DOI: 10.1111/gcb.12219. View

10.

Gow N, Brown A, Odds F . Fungal morphogenesis and host invasion. Curr Opin Microbiol. 2002; 5(4):366-71. DOI: 10.1016/s1369-5274(02)00338-7. View

11.

Silva B, Souza-Goncalves A, Pinto M, Barreto-Bergter E, Santos A . Metallopeptidase inhibitors arrest vital biological processes in the fungal pathogen Scedosporium apiospermum. Mycoses. 2009; 54(2):105-12. DOI: 10.1111/j.1439-0507.2009.01767.x. View

12.

Tammer I, Tintelnot K, Braun-Dullaeus R, Mawrin C, Scherlach C, Schluter D . Infections due to Pseudallescheria/Scedosporium species in patients with advanced HIV disease--a diagnostic and therapeutic challenge. Int J Infect Dis. 2011; 15(6):e422-9. DOI: 10.1016/j.ijid.2011.03.004. View

13.

Wachtler B, Citiulo F, Jablonowski N, Forster S, Dalle F, Schaller M . Candida albicans-epithelial interactions: dissecting the roles of active penetration, induced endocytosis and host factors on the infection process. PLoS One. 2012; 7(5):e36952. PMC: 3351431. DOI: 10.1371/journal.pone.0036952. View

14.

Peeters E, Nelis H, Coenye T . Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Methods. 2007; 72(2):157-65. DOI: 10.1016/j.mimet.2007.11.010. View

15.

Yanagita T . Biochemical aspects on the germination of conidiospores of Aspergillus niger. Arch Mikrobiol. 1957; 26(4):329-44. DOI: 10.1007/BF00407583. View

16.

Kaltseis J, Rainer J, de Hoog G . Ecology of Pseudallescheria and Scedosporium species in human-dominated and natural environments and their distribution in clinical samples. Med Mycol. 2008; 47(4):398-405. DOI: 10.1080/13693780802585317. View

17.

Saracli M, Erdem U, Gonlum A, Yildiran S . Scedosporium apiospermum keratitis treated with itraconazole. Med Mycol. 2003; 41(2):111-4. DOI: 10.1080/mmy.41.2.111.114. View

18.

Gilgado F, Serena C, Cano J, Gene J, Guarro J . Antifungal susceptibilities of the species of the Pseudallescheria boydii complex. Antimicrob Agents Chemother. 2006; 50(12):4211-3. PMC: 1694014. DOI: 10.1128/AAC.00981-06. View

19.

Pinto M, de Sa A, Limongi C, Rozental S, Santos A, Barreto-Bergter E . Involvement of peptidorhamnomannan in the interaction of Pseudallescheria boydii and HEp2 cells. Microbes Infect. 2004; 6(14):1259-67. DOI: 10.1016/j.micinf.2004.07.006. View

20.

Hayer K, Stratford M, Archer D . Structural features of sugars that trigger or support conidial germination in the filamentous fungus Aspergillus niger. Appl Environ Microbiol. 2013; 79(22):6924-31. PMC: 3811532. DOI: 10.1128/AEM.02061-13. View