Integrated Analysis of the Plasmodium Species Transcriptome

Overview

Journal EBioMedicine

Specialty Biomedical Engineering

Date 2016 Jun 21

PMID 27322479

Citations 38

Authors

Regina Hoo

Lei Zhu

Anburaj Amaladoss

Sachel Mok

Onguma Natalang

Stacey A Lapp

Guangan Hu

Kingsley Liew

Mary R Galinski

Zbynek Bozdech

Peter R Preiser

Affiliations

Soon will be listed here.

Abstract

The genome sequence available for different Plasmodium species is a valuable resource for understanding malaria parasite biology. However, comparative genomics on its own cannot fully explain all the species-specific differences which suggests that other genomic aspects such as regulation of gene expression play an important role in defining species-specific characteristics. Here, we developed a comprehensive approach to measure transcriptional changes of the evolutionary conserved syntenic orthologs during the intraerythrocytic developmental cycle across six Plasmodium species. We show significant transcriptional constraint at the mid-developmental stage of Plasmodium species while the earliest stages of parasite development display the greatest transcriptional variation associated with critical functional processes. Modeling of the evolutionary relationship based on changes in transcriptional profile reveal a phylogeny pattern of the Plasmodium species that strictly follows its mammalian hosts. In addition, the work shows that transcriptional conserved orthologs represent potential future targets for anti-malaria intervention as they would be expected to carry out key essential functions within the parasites. This work provides an integrated analysis of orthologous transcriptome, which aims to provide insights into the Plasmodium evolution thereby establishing a framework to explore complex pathways and drug discovery in Plasmodium species with broad host range.

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