Malaria Parasites Regulate Intra-erythrocytic Development Duration Via Serpentine Receptor 10 to Coordinate with Host Rhythms

Overview

Journal Nat Commun

Specialty Biology

Date 2020 Jun 4

PMID 32488076

Citations 29

Authors

Amit K Subudhi

Aidan J ODonnell

Abhinay Ramaprasad

Hussein M Abkallo

Abhinav Kaushik

Hifzur R Ansari

Alyaa M Abdel-Haleem

Fathia Ben Rached

Osamu Kaneko

Richard Culleton

Sarah E Reece

Arnab Pain

Affiliations

Soon will be listed here.

Abstract

Malaria parasites complete their intra-erythrocytic developmental cycle (IDC) in multiples of 24 h suggesting a circadian basis, but the mechanism controlling this periodicity is unknown. Combining in vivo and in vitro approaches utilizing rodent and human malaria parasites, we reveal that: (i) 57% of Plasmodium chabaudi genes exhibit daily rhythms in transcription; (ii) 58% of these genes lose transcriptional rhythmicity when the IDC is out-of-synchrony with host rhythms; (iii) 6% of Plasmodium falciparum genes show 24 h rhythms in expression under free-running conditions; (iv) Serpentine receptor 10 (SR10) has a 24 h transcriptional rhythm and disrupting it in rodent malaria parasites shortens the IDC by 2-3 h; (v) Multiple processes including DNA replication, and the ubiquitin and proteasome pathways, are affected by loss of coordination with host rhythms and by disruption of SR10. Our results reveal malaria parasites are at least partly responsible for scheduling the IDC and coordinating their development with host daily rhythms.

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