Genetically Encoded Optical Activation of DNA Recombination in Human Cells

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2016 Jun 10

PMID 27277957

Citations 27

Authors

J Luo

E Arbely

J Zhang

C Chou

R Uprety

J W Chin

A Deiters

Affiliations

Soon will be listed here.

Abstract

We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.

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