Rapid Blue-light-mediated Induction of Protein Interactions in Living Cells

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2010 Nov 2

PMID 21037589

Citations 541

Authors

Matthew J Kennedy

Robert M Hughes

Leslie A Peteya

Joel W Schwartz

Michael D Ehlers

Chandra L Tucker

Affiliations

Soon will be listed here.

Abstract

Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and subcellular spatial resolution. We demonstrate the utility of this system by inducing protein translocation, transcription and Cre recombinase-mediated DNA recombination using light.

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