Isoxazole-Derived Amino Acids Are Bromodomain-Binding Acetyl-Lysine Mimics: Incorporation into Histone H4 Peptides and Histone H3

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2016 Jun 7

PMID 27264992

Citations 7

Authors

Angelina R Sekirnik Nee Measures

David S Hewings

Natalie H Theodoulou

Lukass Jursins

Katie R Lewendon

Laura E Jennings

Timothy P C Rooney

Tom D Heightman

Stuart J Conway

Affiliations

Soon will be listed here.

Abstract

A range of isoxazole-containing amino acids was synthesized that displaced acetyl-lysine-containing peptides from the BAZ2A, BRD4(1), and BRD9 bromodomains. Three of these amino acids were incorporated into a histone H4-mimicking peptide and their affinity for BRD4(1) was assessed. Affinities of the isoxazole-containing peptides are comparable to those of a hyperacetylated histone H4-mimicking cognate peptide, and demonstrated a dependence on the position at which the unnatural residue was incorporated. An isoxazole-based alkylating agent was developed to selectively alkylate cysteine residues in situ. Selective monoalkylation of a histone H4-mimicking peptide, containing a lysine to cysteine residue substitution (K12C), resulted in acetyl-lysine mimic incorporation, with high affinity for the BRD4 bromodomain. The same technology was used to alkylate a K18C mutant of histone H3.

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