Staphylococcus Aureus Regulatory RNAs As Potential Biomarkers for Bloodstream Infections

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2016 May 26

PMID 27224202

Citations 10

Authors

Valerie Bordeau

Anne Cady

Matthieu Revest

Octavie Rostan

Mohamed Sassi

Pierre Tattevin

Pierre-Yves Donnio

Brice Felden

Affiliations

Soon will be listed here.

Abstract

Staphylococcus aureus is a commensal bacterium and pathogen. Identifying biomarkers for the transition from colonization to disease caused by this organism would be useful. Several S. aureus small RNAs (sRNAs) regulate virulence. We investigated presence and expression of 8 sRNAs in 83 S. aureus strains from 42 patients with sepsis or septic shock and 41 asymptomatic colonized carriers. Small pathogenicity island sRNAs sprB and sprC were clade specific. Six sRNAs had variable expression not correlated with clinical status. Expression of RNAIII was lower in strains from septic shock patients than in strains from colonized patients. When RNAIII was associated with expression of sprD, colonizing strains could be discriminated from strains in patients with bloodstream infections, including patients with sepsis and septic shock. Isolates associated with colonization might have sRNAs with target expression different from those of disease isolates. Monitoring expression of RNAIII and sprD could help determine severity of bloodstream infections.

Citing Articles

Targeted proteomics links virulence factor expression with clinical severity in staphylococcal pneumonia.

Pivard M, Bastien S, Macavei I, Mouton N, Rasigade J, Couzon F Front Cell Infect Microbiol. 2023; 13:1162617.

PMID: 37077532 PMC: 10106754. DOI: 10.3389/fcimb.2023.1162617.

Thirty Years of sRNA-Mediated Regulation in From Initial Discoveries to In Vivo Biological Implications.

Menard G, Silard C, Suriray M, Rouillon A, Augagneur Y Int J Mol Sci. 2022; 23(13).

PMID: 35806357 PMC: 9266662. DOI: 10.3390/ijms23137346.

6S RNA-Dependent Susceptibility to RNA Polymerase Inhibitors.

Esberard M, Hallier M, Liu W, Morvan C, Bossi L, Figueroa-Bossi N Antimicrob Agents Chemother. 2022; 66(5):e0243521.

PMID: 35389235 PMC: 9112884. DOI: 10.1128/aac.02435-21.

Larvae as an Infection Model to Investigate sRNA-Mediated Pathogenesis in .

Menard G, Rouillon A, Ghukasyan G, Emily M, Felden B, Donnio P Front Cell Infect Microbiol. 2021; 11:631710.

PMID: 33954118 PMC: 8089379. DOI: 10.3389/fcimb.2021.631710.

Staphylococcus aureus Responds to Physiologically Relevant Temperature Changes by Altering Its Global Transcript and Protein Profile.

Bastock R, Marino E, Wiemels R, Holzschu D, Keogh R, Zapf R mSphere. 2021; 6(2).

PMID: 33731473 PMC: 8546721. DOI: 10.1128/mSphere.01303-20.

References

Powers M, Bubeck Wardenburg J . Igniting the fire: Staphylococcus aureus virulence factors in the pathogenesis of sepsis. PLoS Pathog. 2014; 10(2):e1003871. PMC: 3923759. DOI: 10.1371/journal.ppat.1003871. View

Foster T, Geoghegan J, Ganesh V, Hook M . Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus. Nat Rev Microbiol. 2013; 12(1):49-62. PMC: 5708296. DOI: 10.1038/nrmicro3161. View

Romilly C, Lays C, Tomasini A, Caldelari I, Benito Y, Hammann P . A non-coding RNA promotes bacterial persistence and decreases virulence by regulating a regulator in Staphylococcus aureus. PLoS Pathog. 2014; 10(3):e1003979. PMC: 3961350. DOI: 10.1371/journal.ppat.1003979. View

Kiehn T, Wong B, Edwards F, Armstrong D . Comparative recovery of bacteria and yeasts from lysis-centrifugation and a conventional blood culture system. J Clin Microbiol. 1983; 18(2):300-4. PMC: 270795. DOI: 10.1128/jcm.18.2.300-304.1983. View

van Belkum A, Verkaik N, de Vogel C, Boelens H, Verveer J, Nouwen J . Reclassification of Staphylococcus aureus nasal carriage types. J Infect Dis. 2009; 199(12):1820-6. DOI: 10.1086/599119. View

Burman J, Leung E, Atkins K, OSeaghdha M, Lango L, Bernado P . Interaction of human complement with Sbi, a staphylococcal immunoglobulin-binding protein: indications of a novel mechanism of complement evasion by Staphylococcus aureus. J Biol Chem. 2008; 283(25):17579-93. PMC: 2649420. DOI: 10.1074/jbc.M800265200. View

Wertheim H, Melles D, Vos M, van Leeuwen W, van Belkum A, Verbrugh H . The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis. 2005; 5(12):751-62. DOI: 10.1016/S1473-3099(05)70295-4. View

Moing V, Alla F, Doco-Lecompte T, Delahaye F, Piroth L, Chirouze C . Staphylococcus aureus Bloodstream Infection and Endocarditis--A Prospective Cohort Study. PLoS One. 2015; 10(5):e0127385. PMC: 4447452. DOI: 10.1371/journal.pone.0127385. View

Keiler K . Mechanisms of ribosome rescue in bacteria. Nat Rev Microbiol. 2015; 13(5):285-97. DOI: 10.1038/nrmicro3438. View

10.

Sassi M, Augagneur Y, Mauro T, Ivain L, Chabelskaya S, Hallier M . SRD: a Staphylococcus regulatory RNA database. RNA. 2015; 21(5):1005-17. PMC: 4408781. DOI: 10.1261/rna.049346.114. View

11.

Hotchkiss R, Monneret G, Payen D . Immunosuppression in sepsis: a novel understanding of the disorder and a new therapeutic approach. Lancet Infect Dis. 2013; 13(3):260-8. PMC: 3798159. DOI: 10.1016/S1473-3099(13)70001-X. View

12.

Smith E, Visai L, Kerrigan S, Speziale P, Foster T . The Sbi protein is a multifunctional immune evasion factor of Staphylococcus aureus. Infect Immun. 2011; 79(9):3801-9. PMC: 3165492. DOI: 10.1128/IAI.05075-11. View

13.

Rosenstein R, Gotz F . What distinguishes highly pathogenic staphylococci from medium- and non-pathogenic?. Curr Top Microbiol Immunol. 2012; 358:33-89. DOI: 10.1007/82_2012_286. View

14.

Dellinger R, Levy M, Rhodes A, Annane D, Gerlach H, Opal S . Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock, 2012. Intensive Care Med. 2013; 39(2):165-228. PMC: 7095153. DOI: 10.1007/s00134-012-2769-8. View

15.

Painter K, Krishna A, Wigneshweraraj S, Edwards A . What role does the quorum-sensing accessory gene regulator system play during Staphylococcus aureus bacteremia?. Trends Microbiol. 2014; 22(12):676-85. DOI: 10.1016/j.tim.2014.09.002. View

16.

Tong S, Davis J, Eichenberger E, Holland T, Fowler Jr V . Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev. 2015; 28(3):603-61. PMC: 4451395. DOI: 10.1128/CMR.00134-14. View

17.

Song J, Lays C, Vandenesch F, Benito Y, Bes M, Chu Y . The expression of small regulatory RNAs in clinical samples reflects the different life styles of Staphylococcus aureus in colonization vs. infection. PLoS One. 2012; 7(5):e37294. PMC: 3358344. DOI: 10.1371/journal.pone.0037294. View

18.

Smyth D, Kafer J, Wasserman G, Velickovic L, Mathema B, Holzman R . Nasal carriage as a source of agr-defective Staphylococcus aureus bacteremia. J Infect Dis. 2012; 206(8):1168-77. PMC: 3448967. DOI: 10.1093/infdis/jis483. View

19.

Rogasch K, Ruhmling V, Pane-Farre J, Hoper D, Weinberg C, Fuchs S . Influence of the two-component system SaeRS on global gene expression in two different Staphylococcus aureus strains. J Bacteriol. 2006; 188(22):7742-58. PMC: 1636327. DOI: 10.1128/JB.00555-06. View

20.

Young B, Golubchik T, Batty E, Fung R, Larner-Svensson H, Votintseva A . Evolutionary dynamics of Staphylococcus aureus during progression from carriage to disease. Proc Natl Acad Sci U S A. 2012; 109(12):4550-5. PMC: 3311376. DOI: 10.1073/pnas.1113219109. View