Time-resolved Resonance Raman Spectroscopy of Bacteriorhodopsin on the Millisecond Timescale

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Dec 1

PMID 271946

Citations 7

Authors

J Terner

A Campion

M A El-Sayed

Affiliations

Soon will be listed here.

Abstract

A simple technique is described that uses a continuous wave laser with electromechanical modulation to obtain time-resolved Raman spectra of transient species on the millisecond timescale. The time behavior of the vibrational bands of the intermediates involved in the proton pumping of bacteriorhodopsin is determined. From these results, along with resonance enhancement and power dependence studies, the bands that appear in the continuous wave Raman spectrum of bacteriorhodopsin can be assigned to three intermediates in the photochemical cycle of bacteriorhodopsin, bR570, bL550, and bM412. The Raman spectra of bR570 and bM412 are compared with published spectra of model Schiff bases of all-trans and 13-cis retinal.

Citing Articles

Time-resolved resonance Raman spectroscopy of intermediates of bacteriorhodopsin: The bK(590) intermediate.

Terner J, Hsieh C, Burns A, El-Sayed M Proc Natl Acad Sci U S A. 1979; 76(7):3046-50.

PMID: 16592669 PMC: 383759. DOI: 10.1073/pnas.76.7.3046.

Experimental evidence for secondary protein-chromophore interactions at the Schiff base linkage in bacteriorhodopsin: Molecular mechanism for proton pumping.

Lewis A, Marcus M, Ehrenberg B, Crespi H Proc Natl Acad Sci U S A. 1978; 75(10):4642-6.

PMID: 16592567 PMC: 336172. DOI: 10.1073/pnas.75.10.4642.

Flash-induced kinetic infrared spectroscopy applied to biochemical systems.

Siebert F, Mantele W, Kreutz W Biophys Struct Mech. 1980; 6(2):139-46.

PMID: 7388122 DOI: 10.1007/BF00535750.

Fourier transform infrared difference spectroscopy of bacteriorhodopsin and its photoproducts.

Bagley K, Dollinger G, Eisenstein L, Singh A, Zimanyi L Proc Natl Acad Sci U S A. 1982; 79(16):4972-6.

PMID: 6956906 PMC: 346807. DOI: 10.1073/pnas.79.16.4972.

Determination of retinal chromophore structure in bacteriorhodopsin with resonance Raman spectroscopy.

Smith S, Lugtenburg J, Mathies R J Membr Biol. 1985; 85(2):95-109.

PMID: 4009698 DOI: 10.1007/BF01871263.

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